Product info: FalI Name FalI Cat. # E153 E154 Package, u.a. 100 500 Concentration, u.a./ml 1000-3000 1000-3000 Recognition site ↑(N)8AAGNNNNNCTT(N)13↑ ↓(N)13TTCNNNNNGAA(N)8↓ Source Flavobacterium aquatile Ob10 Assayed on Lambda DNA Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. Optimal SE-buffer W (10 mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) + SAM Enzyme activity (%) B G O W Y Rose 0 - 10 25 - 50 75 - 100 100 50 - 75 70 Optimal temperature 37oC Storage conditions 10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C. Ligations After 3-fold overdigestion with enzyme 20% of the DNA fragments can be ligated and of these 80% can be recut. In the presence of 10%PEG ligation is better. Non-specific hydrolisis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 37°C. Reagents Supplied with Enzyme 10 X SE-buffer W, SAM Methylation sensitivity not tested Inactivation 20 minutes under 65oC Notes High enzyme concentration results in star activity To obtain 100% activity, SAM should be added to a final concentration 0.01 mM Quality control Restriction Endonucleases: Quality Control MSDS: Download MSDS as PDF References: Dedkov, V.S., Sinichkina, S.A., Abdurashitov, M.A., Popichenko, D.V., Degtyarev, S.K. Unpublished observations (2001).