Product info: FatI Name FatI      Cat. # E155 E156 Package, u.a. 100 500 Concentration, u.a./ml 5000 5000 Recognition site ↑CATG GTAC↓ Source An E.coli strain that carries the cloned Fat I gene from Flavobacterium aquatile NL3 Assayed on pUC19 DNA Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of pUC19 DNA in 1 hour at 55°C in a total reaction volume of 50 μl. Optimal SE-buffer G (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.) Enzyme activity (%) B G O W Y Rose 10 - 25 100 25 - 50 10 - 25 50 - 75 100 Optimal temperature 55oC Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0,1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; and 50% glycerol. Store at -20°C. Ligation After 2-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut. Non-specific hydrolisis No nonspecific activity was detected after incubation of 1 μg of DNA with 3 u.a. of enzyme for 16 hours at 55°C. Reagents Supplied with Enzyme 10 X SE-buffer G Methylation sensitivity Blocked by mCATG methylation Inactivation 20 minutes under 65oC Notes The minimum number of units that resulted in complete digestion of 1 μg of substrate DNA in 16 hours is 0,25. FatI cleaves linear plasmid DNA at a rate 1.5-2 times higher than supercoiled plasmid DNA. Quality control Restriction Endonucleases: Quality Control MSDS: Download MSDS as PDF References: Dedkov, V.S., Kileva, E.V., Popichenko, D.V., Degtyarev, S.K. Biotekhnologia #5, 3-7 (2002)  V.A. Chernukhin, M.A. Abdurashitov, V.N. Tomilov, D.A. Gonchar, S.Kh. Degtyarev Comparative restriction enzymes analysis of rat chromosomal DNA in vitro and in silico // "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.2, No 3, pp 39-46, 2006