Product info: DNA Quick Ligation Kit


Name
DNA Quick Ligation Kit
Cat. #K006
Package, reactions20

DescriptionPackage - 20 reactions
The Quick Ligation Kit includes:
1. Quick T4 DNA Ligase (recombinant, 2000 u/μl) - 20μl
2. 2 x Quick Ligation Buffer - 200μl
(132 mM Tris-HCI (pH 7.6 at 25°C); 20 mM MgCI2, 2 mM DTT; 2 mM ATP; 15% PEG 6000).
Mix thoroughly before use.
Storage conditionsStore at -20°C.
NotesLigation reaction protocol ( for 20 μl )
1. Mix:
      H2O - calculated quantity
      2 x Quick Ligation Buffer - 10μl
      Add DNA
      Add 1 μl Quick T4 DNA Ligase
2. Incubate at room temperature (+25°C) for 5 min.
3. Cool the mixture on ice, store at -20°C.

Notes on Use: Before the first use the 2 x SE-DNA Quick Ligation Buffer should be divided into small aliquots and store at -20°C.
Avoid defrosting this Buffer more than 2-3 times.
The Buffer can be stored at +4°C during 7 days.
The efficiency of ligation starts to decrease after 2 hours of incubation and is reduced by up to 75% if the reaction is allowed to go overnight at 25°C.
Before using the products of a Quick Ligation reaction for electrotransformation, it is necessary to reduce the PEG concentration.