|Product info: DNA Quick Ligation Kit|
|Name||DNA Quick Ligation Kit|
|Cat. #||K006 |
|Package, reactions||20 |
|Description||Package - 20 reactions|
The Quick Ligation Kit includes:
1. Quick T4 DNA Ligase (recombinant, 2000 u/μl) - 20μl
2. 2 x Quick Ligation Buffer - 200μl
(132 mM Tris-HCI (pH 7.6 at 25°C); 20 mM MgCI2, 2 mM DTT; 2 mM ATP; 15% PEG 6000).
Mix thoroughly before use.
|Storage conditions||Store at -20°C.|
|Notes||Ligation reaction protocol ( for 20 μl ) |
H2O - calculated quantity
2 x Quick Ligation Buffer - 10μl
Add 1 μl Quick T4 DNA Ligase
2. Incubate at room temperature (+25°C) for 5 min.
3. Cool the mixture on ice, store at -20°C.
Notes on Use:
Before the first use the 2 x SE-DNA Quick Ligation Buffer should be divided into small aliquots and store at -20°C.
Avoid defrosting this Buffer more than 2-3 times.
The Buffer can be stored at +4°C during 7 days.
The efficiency of ligation starts to decrease after 2 hours of incubation and is reduced by up to 75% if the reaction is allowed to go overnight at 25°C.
Before using the products of a Quick Ligation reaction for electrotransformation, it is necessary to reduce the PEG concentration.