Product info: DNA Quick Ligation Kit |
Name | DNA Quick Ligation Kit | Cat. # | K006 | Package, reactions | 20 |
Description | Package - 20 reactions
The Quick Ligation Kit includes:
1. Quick T4 DNA Ligase (recombinant, 2000 u/μl) - 20μl
2. 2 x Quick Ligation Buffer - 200μl
(132 mM Tris-HCI (pH 7.6 at 25°C); 20 mM MgCI2, 2 mM DTT; 2 mM ATP; 15% PEG 6000).
Mix thoroughly before use.
| Storage conditions | Store at -20°C. | Notes | Ligation reaction protocol ( for 20 μl )
1. Mix:
H2O - calculated quantity
2 x Quick Ligation Buffer - 10μl
Add DNA
Add 1 μl Quick T4 DNA Ligase
2. Incubate at room temperature (+25°C) for 5 min.
3. Cool the mixture on ice, store at -20°C.
Notes on Use:
Before the first use the 2 x SE-DNA Quick Ligation Buffer should be divided into small aliquots and store at -20°C.
Avoid defrosting this Buffer more than 2-3 times.
The Buffer can be stored at +4°C during 7 days.
The efficiency of ligation starts to decrease after 2 hours of incubation and is reduced by up to 75% if the reaction is allowed to go overnight at 25°C.
Before using the products of a Quick Ligation reaction for electrotransformation, it is necessary to reduce the PEG concentration. |
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