Product info: DNA Quick Ligation Kit Name DNA Quick Ligation Kit Cat. # K006 Package, reactions 20 Description Package - 20 reactions The Quick Ligation Kit includes: 1. Quick T4 DNA Ligase (recombinant, 2000 u/μl) - 20μl 2. 2 x Quick Ligation Buffer - 200μl (132 mM Tris-HCI (pH 7.6 at 25°C); 20 mM MgCI2, 2 mM DTT; 2 mM ATP; 15% PEG 6000). Mix thoroughly before use. Storage conditions Store at -20°C. Notes Ligation reaction protocol ( for 20 μl ) 1. Mix:       H2O - calculated quantity       2 x Quick Ligation Buffer - 10μl       Add DNA       Add 1 μl Quick T4 DNA Ligase 2. Incubate at room temperature (+25°C) for 5 min. 3. Cool the mixture on ice, store at -20°C. Notes on Use: Before the first use the 2 x SE-DNA Quick Ligation Buffer should be divided into small aliquots and store at -20°C. Avoid defrosting this Buffer more than 2-3 times. The Buffer can be stored at +4°C during 7 days. The efficiency of ligation starts to decrease after 2 hours of incubation and is reduced by up to 75% if the reaction is allowed to go overnight at 25°C. Before using the products of a Quick Ligation reaction for electrotransformation, it is necessary to reduce the PEG concentration.