Product info: Bmt I


Name
Bmt I
Cat. #E457E458
Package, u.a.10005000
Concentration, u.a./ml2000020000

Recognition site
GCTAGC
CGATCG
SourceE.coli strain that carries the cloned Bmt I gene from Bacillus megaterium S2
Assayed onLambda DNA (Hind III-digest)
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (Hind III-digest) in 1 hour at 37°C in a total reaction volume of 50 μl.
Optimal SE-bufferW (10 mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.)
Enzyme activity (%)
BGOWYRose
10 - 2550 - 7550 - 7510075 - 100100
Optimal temperature
37oC
Storage conditions10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
LigationsAfter 20-fold overdigestion with enzyme about 95% of the DNA fragments can be ligated and recut.
Non-specific hydrolisisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C.
Reagents Supplied with Enzyme 10 X SE-buffer W
Methylation sensitivitynot tested
Inactivation 20 minutes under
65oC
NotesThe minimum number of units that resulted in complete digestion of 1 μg of substrate DNA in 16 hours is 0,13. BmtI cleaves linear plasmid DNA at a rate 5 times higher than supercoiled plasmid DNA.
Quality control
Restriction Endonucleases: Quality Control
MSDS:Download MSDS as PDF
References:Dedkov, V.S., Nayakshina, T.N., Popichenko, D.V., Degtyarev, S.K. Biotekhnologiya 1: 11-15 (2003).
Theoretical diagrams of DNA digestion by this enzyme for the most known DNA substrates:

To view the fragments length values please point mouse cursor over diagram
Fragment lengths
M12345M

M - ladder, 1 - Adeno-2 DNA, 2 - Lambda DNA, 3 - T7 DNA, 4 - pUC19, 5 - pBR322