|Source||An E.coli strain that carries the cloned Bmt I gene from Bacillus megaterium S2|
|Assayed on||Lambda DNA (Hind III-digest)|
|Unit definition||One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (Hind III-digest) in 1 hour at 37°C in a total reaction volume of 50 μl.|
|Optimal SE-buffer||W (10 mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.)|
|Enzyme activity (%)|
|10 - 25||50 - 75||50 - 75||100||75 - 100||100|
|Storage conditions||10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol; Store at -20°C.|
|Ligation||After 20-fold overdigestion with enzyme about 95% of the DNA fragments can be ligated and recut.|
|Non-specific hydrolisis||No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C.
|Reagents Supplied with Enzyme||
10 X SE-buffer W
|Methylation sensitivity||not tested|
|Inactivation 20 minutes under||65oC|
|Notes||The minimum number of units that resulted in complete digestion of 1 μg of substrate DNA in 16 hours is 0,13.
BmtI cleaves linear plasmid DNA at a rate 5 times higher than supercoiled plasmid DNA.
|Quality control||Restriction Endonucleases: Quality Control|
|MSDS:||Download MSDS as PDF|
|References:||Dedkov, V.S., Nayakshina, T.N., Popichenko, D.V., Degtyarev, S.K.
Biotekhnologiya 1: 11-15 (2003).|
|Theoretical diagrams of DNA digestion by this enzyme for the most known DNA substrates:|
To view the fragments length values please point mouse cursor over diagram
M - ladder, 1 - Adeno-2 DNA, 2 - Lambda DNA, 3 - T7 DNA, 4 - pUC19, 5 - pBR322