|Source||Bacillus stearothermophilus MW|
|Assayed on||Lambda DNA|
|Unit definition||One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 55°C in a total reaction volume of 50 μl.|
|Optimal SE-buffer||Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)|
|Enzyme activity (%)|
|10 - 25||25 - 50||25 - 50||50 - 75||100||40|
|Storage conditions||10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol.|
Storage at -20°C.
Storage at -70°C is recommended for periods longer than 7 days.
|Ligation||After 5-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.|
|Non-specific hydrolisis||No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 55°C.
|Reagents Supplied with Enzyme||
10 X SE-buffer Y|
|Methylation sensitivity||not tested|
|Inactivation 20 minutes under||80oC|
|Notes||At 37°C activity is 20% from maximum
|Quality control||Restriction Endonucleases: Quality Control|
|MSDS:||Download MSDS as PDF|
|References:||Nadeev A.N., Dedkov, V.S., Tomilova J.E., Degtyarev, S.K. Unpublished observations (2002). |