Product info: Hga I

Hga IBlocked by 
GA(5mC)GC methylation  
Cat. #E461E462
Package, u.a.50250
Concentration, u.a./ml10001000

Recognition site
SourceAn E.coli strain that carries the cloned HgaI gene from Haemophilus gallinarum
Assayed onDNA pBR322
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of DNA pBR322 in 1 hour at 37°C in a total reaction volume of 50 μl.
Optimal SE-bufferB (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 1 mM DTT.)
Enzyme activity (%)
10075 - 10010 - 2525 - 5050 - 7550
Optimal temperature
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
LigationAfter 3-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisisIncubation with > 2 units of HgaI per 1 μg of DNA and digestion > 1 hour are not recommended.
Reagents Supplied with Enzyme 10 X SE-buffer B
Methylation sensitivityBlocked by CG methylation.
Inactivation 20 minutes under
Quality control
Restriction Endonucleases: Quality Control
MSDS:Download MSDS as PDF
References:Brown N.L., Smith M. Proc. Natl. Acad. Sci. U. S. A. 74: 3213-3216 (1977). Sugisaki H. Gene 3: 17-28 (1978).