Product info: Hga I


Name
Hga IBlocked by 
GA(5mC)GC methylation  
Cat. #E461E462
Package, u.a.50250
Concentration, u.a./ml10001000

Recognition site
GACGC(N)5
CTGCG(N)10
SourceE.coli strain that carries the cloned HgaI gene from Haemophilus gallinarum
Assayed onDNA pBR322
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of DNA pBR322 in 1 hour at 37°C in a total reaction volume of 50 μl.
Optimal SE-bufferB (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 1 mM DTT.)
Enzyme activity (%)
BGOWYRose
10075 - 10010 - 2525 - 5050 - 7550
Optimal temperature
37oC
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
LigationsAfter 3-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisisIncubation with > 2 units of HgaI per 1 μg of DNA and digestion > 1 hour are not recommended.
Reagents Supplied with Enzyme 10 X SE-buffer B
Methylation sensitivityBlocked by CG methylation.
Inactivation 20 minutes under
65oC
Quality control
Restriction Endonucleases: Quality Control
MSDS:Download MSDS as PDF
References:Brown N.L., Smith M. Proc. Natl. Acad. Sci. U. S. A. 74: 3213-3216 (1977). Sugisaki H. Gene 3: 17-28 (1978).
Theoretical diagrams of DNA digestion by this enzyme for the most known DNA substrates:

To view the fragments length values please point mouse cursor over diagram
Fragment lengths
M12345M

M - ladder, 1 - Adeno-2 DNA, 2 - Lambda DNA, 3 - T7 DNA, 4 - pUC19, 5 - pBR322