Product info: BpmI Name BpmI    Cat. # E467 E468 Package, u.a. 50 250 Concentration, u.a./ml 500 - 1000 500 - 1000 Recognition site CTGGAG(N)16↑ GACCTC(N)14↓ Source Bacillus pumilus Assayed on Lambda DNA Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. Optimal SE-buffer W (10 mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) + BSA Enzyme activity (%) B G O W Y Rose 25 - 50 50 - 75 75 - 100 100 50 - 75 100 Optimal temperature 37oC Storage conditions 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Store at -20°C Ligation After 2-fold overdigestion with enzyme about 95% of the DNA fragments can be ligated and 95% may be recut. Non-specific hydrolisis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 2 u.a. of enzyme for 16 hours at 37°C. Reagents Supplied with Enzyme 10 X SE-buffer W, BSA Methylation sensitivity Blocked by overlapping Dcm-methylation (CmCWGG): 5'- C(5mC)TGGAG(N)16-3' 3'- GGA(5mC)CTC(N)14-5' Inactivation 20 minutes under 65oC Notes To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 μg/ml. Do not use BSA for long incubation. Quality control Restriction Endonucleases: Quality Control MSDS: Download MSDS as PDF References: Degtyarev, S.Kh., Morgan P. Unpublished observation (1992).