Product info: BpmI


Name
BpmIBlocked by   
 DCM methylation  
Cat. #E467E468
Package, u.a.50250
Concentration, u.a./ml500 - 1000500 - 1000

Recognition site
CTGGAG(N)16
GACCTC(N)14
SourceBacillus pumilus
Assayed onLambda DNA
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Optimal SE-bufferW (10 mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) + BSA
Enzyme activity (%)
BGOWYRose
25 - 5050 - 7575 - 10010050 - 75100
Optimal temperature
37oC
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Store at -20°C
LigationAfter 2-fold overdigestion with enzyme about 95% of the DNA fragments can be ligated and 95% may be recut.
Non-specific hydrolisisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 2 u.a. of enzyme for 16 hours at 37°C.
Reagents Supplied with Enzyme 10 X SE-buffer W, BSA
Methylation sensitivityBlocked by overlapping Dcm-methylation (CmCWGG):
5'- C(5mC)TGGAG(N)16-3'
3'- GGA(5mC)CTC(N)14-5'
Inactivation 20 minutes under
65oC
NotesTo obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 μg/ml.
Do not use BSA for long incubation.
Quality control
Restriction Endonucleases: Quality Control
MSDS:Download MSDS as PDF
References:Degtyarev, S.Kh., Morgan P. Unpublished observation (1992).