Product info: Hpa I


Name
Hpa I
Cat. #E077E078E077TE078TE077m
Package, u.a.50025005002500250
Concentration, u.a./ml50005000500050005000

Recognition site
GTTAAC
CAATTG
SourceE.coli strain, that carries the cloned gene Hpa I from Haemophilus parainfluenzae
Assayed onLambda DNA
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Optimal SE-bufferY (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)
Enzyme activity (%)
BGOWYRose
0 - 1050 - 7510 - 2525 - 5010025
Optimal temperature
37oC
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
LigationsAfter 5-fold overdigestion with enzyme about 60% of the DNA fragments can be ligated and recut.
Non-specific hydrolisisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 37°C.
Reagents Supplied with Enzyme 10 X SE-buffer Y.
For E077T and E078T an additional 10X SE-buffer ROSE is supplied.
Methylation sensitivitynot tested
Inactivation 20 minutes under
65oC
NotesHigh enzyme concentration results in star activity.
Quality control
Restriction Endonucleases: Quality Control
MSDS:Download MSDS as PDF
References:Sharp, P.A., Sugden, B., Sambrook, J. Biochemistry 12: 3055-3063 (1973). Garfin, D.E., Goodman, H.M. Biochem. Biophys. Res. Commun. 59: 108-116 (1974).
Theoretical diagrams of DNA digestion by this enzyme for the most known DNA substrates:

To view the fragments length values please point mouse cursor over diagram
Fragment lengths
M12345M

M - ladder, 1 - Adeno-2 DNA, 2 - Lambda DNA, 3 - T7 DNA, 4 - pUC19, 5 - pBR322