Recognition site | GTT↑AAC CAA↓TTG |
Source | An E.coli strain, that carries the cloned gene Hpa I from Haemophilus parainfluenzae |
Assayed on | Lambda DNA |
Unit definition | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. |
Optimal SE-buffer | Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) |
Enzyme activity (%) | B | G | O | W | Y | Rose | 0 - 10 | 50 - 75 | 10 - 25 | 25 - 50 | 100 | 25 |
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Optimal temperature | 37oC |
Storage conditions | 10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol. Store at -20°C. |
Ligation | After 5-fold overdigestion with enzyme about 60% of the DNA fragments can be ligated and recut. |
Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 5 u.a. of enzyme for 16 hours at 37°C.
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Reagents Supplied with Enzyme |
10 X SE-buffer Y. |
Methylation sensitivity | not tested |
Inactivation 20 minutes under | 65oC |
Notes | High enzyme concentration results in star activity.
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Quality control | Restriction Endonucleases: Quality Control |
MSDS: | Download MSDS as PDF |
References: | Sharp, P.A., Sugden, B., Sambrook, J. Biochemistry 12: 3055-3063 (1973).
Garfin, D.E., Goodman, H.M. Biochem. Biophys. Res. Commun. 59: 108-116 (1974).
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