Product info: EcoICR I


Name
EcoICR I
Cat. #E469E470
Package, u.a.2001000
Concentration, u.a./ml2000-100002000-10000

Recognition site
GAGCTC
CTCGAG
SourceEscherichia coli ICR
Assayed onLambda DNA (HindIII-digest)
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (HindIII-digest) in 1 hour at 37°C in a total reaction volume of 50 μl.
Optimal SE-bufferG (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.) + BSA
Enzyme activity (%)
BGOWYRose
75 - 1001000 - 100 - 1075 - 1005
Optimal temperature
37oC
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol; Store at -20°C.
LigationsAfter 5-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut.
Non-specific hydrolisisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C.
Reagents Supplied with Enzyme 10 X SE-buffer G, BSA
Methylation sensitivitynot tested
Inactivation 20 minutes under
65oC
NotesTo obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml
Do not use BSA for long incubation.
Quality control
Restriction Endonucleases: Quality Control
MSDS:Download MSDS as PDF
References:Sugisaki H., Maekawa Y., Kanazawa S., Takanami M. Bull. Inst. Chem. Res. Kyoto Univ. 60: 328-335 (1982). Yankovsky N.K., Fonstein M.Yu., Lashina S.Yu., Bukanov N.O., Yakubovich N.V., Ermakova L.M., Rebentish B.A., Janulaitis A., Debabov V.G. Gene 81: 203-210 (1989).
Theoretical diagrams of DNA digestion by this enzyme for the most known DNA substrates:

To view the fragments length values please point mouse cursor over diagram
Fragment lengths
M12345M

M - ladder, 1 - Adeno-2 DNA, 2 - Lambda DNA, 3 - T7 DNA, 4 - pUC19, 5 - pBR322