Product info: AsiG I


Name
AsiG I
Cat. #E235E236
Package, u.a.100500
Concentration, u.a./ml3000-50003000-5000

Recognition site
ACCGGT
TGGCCA
SourceArthrobacter species G
Assayed onLambda DNA
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Optimal SE-bufferO (50 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.)
Enzyme activity (%)
BGOWYRose
10 - 2525 - 5010075 - 10010 - 2575
Optimal temperature
37oC
Storage conditions10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA; 50% glycerol; Store at -20°C.
LigationAfter 5-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C.
Reagents Supplied with Enzyme 10 X SE-buffer O.
Methylation sensitivitynot tested
Inactivation 20 minutes under
65oC
NotesTurbo AsiG I can be used for short time (10 min) DNA digestion as well as for standard reaction. The reaction can be performed using optimal or universal (ROSE) Buffer.
Quality control
Restriction Endonucleases: Quality Control
MSDS:Download MSDS as PDF
References:Chmuzh, E.V., Mikhnenkova, N.A., Nayakshina, T.N., Mezentseva, N.V., Dedkov, V.S., Degtyarev, S.K. Unpublished observations.(2005)
Theoretical diagrams of DNA digestion by this enzyme for the most known DNA substrates:

To view the fragments length values please point mouse cursor over diagram
Fragment lengths
M12345M

M - ladder, 1 - Adeno-2 DNA, 2 - Lambda DNA, 3 - T7 DNA, 4 - pUC19, 5 - pBR322