Product info: AspLE I Name AspLE I      Cat. # E221 E222 Package, u.a. 500 2500 Concentration, u.a./ml 10000 10000 Recognition site GCG↑C C↓GCG Source Arthrobacter species LE3860 Assayed on Lambda DNA Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. Optimal SE-buffer O (50 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) Enzyme activity (%) B G O W Y Rose 10 - 25 75 - 100 100 50 - 75 25 - 50 100 Optimal temperature 37oC Storage conditions 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA; 50% glycerol. Store at -20°C. Ligations After 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut. Non-specific hydrolisis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C. Reagents Supplied with Enzyme 10 X SE-buffer O Methylation sensitivity Blocked by 5'-G(5mC)GC-3'/3-CG(5mC)G-5' methylation. Not blocked by 5'-GCG(5mC)-3'/3`-(5mC)GCG-5` or 5'-GCG(5mC)-3'/3'-CGCG-5' methylation. Cut hemimethylated site: 5'-G(5mC)GC-3'/3'-CGCG-5' Inactivation 20 minutes under 80oC Quality control Restriction Endonucleases: Quality Control MSDS: Download MSDS as PDF References: Vysotskaya, E.M., Gonchar, D.A., Shevchenko, A.V., Abdurashitov, M.A., Degtyarev, S.Kh. Unpublished observations (1998). Theoretical diagrams of DNA digestion by this enzyme for the most known DNA substrates: To view the fragments length values please point mouse cursor over diagram Fragment lengths M 1 2 3 4 5 M M - ladder, 1 - Adeno-2 DNA, 2 - Lambda DNA, 3 - T7 DNA, 4 - pUC19, 5 - pBR322