Product info: AspLE I


Name
AspLE IBlocked by 
5`-G(5mC)GC-3`/3-CG(5mC)G-5` or 
5`-G(5mC)GC-3`/3`-CGCG-5`
methylation   Not blocked by 
5`-GCG(5mC)-3`/3`-(5mC)GCG-5` or 
5`-GCG(5mC)-3`/3`-CGCG-``
methylation   
Cat. #E221E222
Package, u.a.5002500
Concentration, u.a./ml10000-3000010000-30000

Recognition site
GCGC
CGCG
SourceArthrobacter species LE3860
Assayed onLambda DNA
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Optimal SE-bufferO (50 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.)
Enzyme activity (%)
BGOWYRose
10 - 2575 - 10010050 - 7525 - 50100
Optimal temperature
37oC
Storage conditions10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA; 50% glycerol. Store at -20°C.
LigationsAfter 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C.
Reagents Supplied with Enzyme 10 X SE-buffer O
Methylation sensitivityBlocked by
5'-G(5mC)GC-3'/3-CG(5mC)G-5' or
5'-G(5mC)GC-3'/3'-CGCG-5' methylation.
Not blocked by
5'-GCG(5mC)-3'/3`-(5mC)GCG-5` or
5'-GCG(5mC)-3'/3'-CGCG-5' methylation.
Cut hemimethylated site: 5'-G(5mC)GC-3'/3'-CGCG-5'
Inactivation 20 minutes under
80oC
Quality control
Restriction Endonucleases: Quality Control
MSDS:Download MSDS as PDF
References:Vysotskaya, E.M., Gonchar, D.A., Shevchenko, A.V., Abdurashitov, M.A., Degtyarev, S.Kh. Unpublished observations (1998).
Theoretical diagrams of DNA digestion by this enzyme for the most known DNA substrates:

To view the fragments length values please point mouse cursor over diagram
Fragment lengths
M12345M

M - ladder, 1 - Adeno-2 DNA, 2 - Lambda DNA, 3 - T7 DNA, 4 - pUC19, 5 - pBR322