| Recognition site | GCG↑C
C↓GCG |
| Source | Arthrobacter species LE3860 |
| Assayed on | Lambda DNA |
| Unit definition | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. |
| Optimal SE-buffer | O (50 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) |
| Enzyme activity (%) | | B | G | O | W | Y | Rose | | 10 - 25 | 75 - 100 | 100 | 50 - 75 | 25 - 50 | 100 |
|
| Optimal temperature | 37oC |
| Storage conditions | 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA; 50% glycerol. Store at -20°C. |
| Ligation | After 10-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut. |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C.
|
| Reagents Supplied with Enzyme |
10 X SE-buffer O
|
| Methylation sensitivity | Blocked by
5'-G(5mC)GC-3'/3-CG(5mC)G-5'
methylation.
Not blocked by
5'-GCG(5mC)-3'/3`-(5mC)GCG-5` or
5'-GCG(5mC)-3'/3'-CGCG-5'
methylation.
Cut hemimethylated site: 5'-G(5mC)GC-3'/3'-CGCG-5' |
| Inactivation 20 minutes | No |
| Quality control | Restriction Endonucleases: Quality Control |
| MSDS: | Download MSDS as PDF |
| References: | Vysotskaya, E.M., Gonchar, D.A., Shevchenko, A.V., Abdurashitov, M.A., Degtyarev, S.Kh. Unpublished observations (1998).
|
