Product info: AspS9 I


Name
AspS9 IBlocked by 
GGNC(5mC) methylation    
Cat. #E117E118
Package, u.a.10005000
Concentration, u.a./ml10000-3000010000-30000

Recognition site
GGNCC
CCNGG
SourceArthrobacter species S9
Assayed onLambda DNA
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Optimal SE-bufferW (10 mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.)
Enzyme activity (%)
BGOWYRose
50 - 7550 - 7575 - 10010050 - 7575
Optimal temperature
37oC
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
LigationsAfter 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 30 u.a. of enzyme for 16 hours at 37°C.
Reagents Supplied with Enzyme 10 X SE-buffer W
Methylation sensitivityBloked by overlapping Dcm methylation(CmCWGG): GGNCCWGG
Inactivation 20 minutes under
65oC
Quality control
Restriction Endonucleases: Quality Control
MSDS:Download MSDS as PDF
References:Dedkov, V.S., Kileva, E.V., Shevchenko, A.V., Degtyarev, S.Kh. Unpublished observations (1995).
 Chernukhin V.A, Abdurashitov M.A., Tomilov V.N., Gonchar D.A., Degtyarev S.Kh. Comparative analysis of mouse chromosomal DNA digestion with restriction endonucleases in vitro and in silico // "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.3, No 4, pp 19-27, 2007
 V.A. Chernukhin, M.A. Abdurashitov, V.N. Tomilov, D.A. Gonchar, S.Kh. Degtyarev Comparative restriction enzymes analysis of rat chromosomal DNA in vitro and in silico // "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.2, No 3, pp 39-46, 2006