Product info: N.Bst9 I


Name
N.Bst9 I
Cat. #E401E402
Package, u.a.100500
Concentration, u.a./ml2000-50002000-5000

Recognition site
GAGTC(4/-)
SourceBacillus stearothermophillus T9
DescriptionN.Bst9I is a site specific DNA endonuclease that cleaves only one strand of double-stranded DNA substrate.
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of T7 DNA in 1 hour at 55C in a total reaction volume of 50μl. Concentrated enzymes are diluted to approximately 1000 units/ml with the buffer [10 mM Tris-HCl (pH 7.6); 50 mM KCl; 0.1 mM EDTA; 1 mM DTT; 200 μg/ml BSA; 50% glycerol] before determining their activity.
Reaction bufferSE-buffer N.Bst9I, (10 mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl2; 150 mM KCl; 1 mM DTT.)
Optimal temperature
55oC
Storage conditions10 mM Tis-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Store at -20C.
LigationsAfter 5-fold overdigestion with N.Bst9 I, 90% of the T7 DNA fragments can be ligated and recut.
Non-specific hydrolisisNo nonspecific activity was detected after incubation of 1 μg of T7 DNA with 2 units of N.Bst9I for 16 hours.
Reagents Supplied with Enzyme 10 X SE-buffer N.Bst9I
Inactivation 20 minutes under
80oC
NotesHigh enzyme concentration and non optimal reaction conditions may result in star activity.
Incubation at 37C results in 20% activity.
MSDS:Download MSDS as PDF