Product info: BamH I


Name
BamH INot blocked by 
 GG(6mA)TCC methylation   
Cat. #E021E022E021XE022XE021TE022TE021m
Package, u.a.400020000400020000400020000500
Concentration, u.a./ml20000200005000050000200002000010000

Recognition site
GGATCC
CCTAGG
SourceE.coli strain, that carries the cloned gene BamHI from Bacillus amyloliquefaciens H
Assayed onLambda DNA
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Optimal SE-bufferG (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.) + BSA
Enzyme activity (%)
BGOWYRose
25 - 5010075 - 10075 - 10025 - 50100
Optimal temperature
37oC
Storage conditions10 mM Tris-HCl (pH 7.6); 50 mM NaCl; 0,1 mM EDTA; 100 μg/ml BSA; 1 mM DTT; 50% glycerol. Store at -20°C.
LigationsAfter 50-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 37°C.
Reagents Supplied with Enzyme 10 X SE-buffer G, BSA (except E021m, E021T and E022T).
For E021T and E022T an additional 10X SE-buffer ROSE is supplied.
Methylation sensitivityNot blocked by Dam methylation (GmATC) GGATCC
Inactivation 20 minutes under
65oC
NotesHigh enzyme concentration may result in star activity.
To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 μg/ml.
Do not use BSA for long incubation.
Quality control
Restriction Endonucleases: Quality Control
MSDS:Download MSDS as PDF
References:Wilson, G.A. and Young, F.E. J. Mol. Biol. 97: 123-125 (1975). Roberts, R.J., Wilson, G.A., Young, F.E. Nature 265: 82-84 (1977)
Theoretical diagrams of DNA digestion by this enzyme for the most known DNA substrates:

To view the fragments length values please point mouse cursor over diagram
Fragment lengths
M12345M

M - ladder, 1 - Adeno-2 DNA, 2 - Lambda DNA, 3 - T7 DNA, 4 - pUC19, 5 - pBR322