|Source||An E.coli strain, that carries the cloned gene BamHI from Bacillus amyloliquefaciens H|
|Assayed on||Lambda DNA|
|Unit definition||One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.|
|Optimal SE-buffer||G (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.) + BSA|
|Enzyme activity (%)|
|25 - 50||100||75 - 100||75 - 100||25 - 50||100|
|Storage conditions||10 mM Tris-HCl (pH 7.6); 50 mM NaCl; 0,1 mM EDTA; 100 μg/ml BSA; 1 mM DTT; 50% glycerol. Store at -20°C.|
|Ligation||After 50-fold overdigestion with enzyme approximately 90% of the DNA fragments can be ligated and recut.|
|Non-specific hydrolisis||No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C.
|Reagents Supplied with Enzyme||
10 X SE-buffer G, BSA
|Methylation sensitivity||Not blocked by Dam methylation (GmATC) GGATCC|
|Inactivation 20 minutes under||65oC|
|Notes||High enzyme concentration may result in star activity.|
To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 μg/ml.
Do not use BSA for long incubation.
|Quality control||Restriction Endonucleases: Quality Control|
|MSDS:||Download MSDS as PDF|
|References:||Wilson, G.A. and Young, F.E. J. Mol. Biol. 97: 123-125 (1975).
Roberts, R.J., Wilson, G.A., Young, F.E. Nature 265: 82-84 (1977)|
|Theoretical diagrams of DNA digestion by this enzyme for the most known DNA substrates:|
To view the fragments length values please point mouse cursor over diagram
M - ladder, 1 - Adeno-2 DNA, 2 - Lambda DNA, 3 - T7 DNA, 4 - pUC19, 5 - pBR322