Product info: Bgl I Name Bgl I      Cat. # E025 E026 Package, u.a. 500 2500 Concentration, u.a./ml 5000-10000 5000-10000 Recognition site GCCNNNN↑NGGC CGGN↓NNNNCCG Source Bacillus globigii Assayed on Lambda DNA Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. Optimal SE-buffer 2W (20 mM Tris-HCl (pH 8,5 at 25°C); 10 mM MgCl2; 200 mM NaCl; 1 mM DTT) Enzyme activity (%) B G O W Y Rose 50 - 75 50 - 75 0 - 10 75 - 100 25 - 50 100 Optimal temperature 37oC Storage conditions 10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Store at -20°C. Ligation After 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut. Non-specific hydrolisis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C. Reagents Supplied with Enzyme 10 X SE-buffer 2W Methylation sensitivity Not blocked by dcm methylation (CmCWGG): GCCWGGNNGGC Inactivation 20 minutes under 65oC Notes High enzyme concentration may result in star activity Quality control Restriction Endonucleases: Quality Control MSDS: Download MSDS as PDF References: Duncan, C.H., Wilson, G.A. and Young, F.E. J. Bacteriol. 134: 338 (1978).