Product info: Bgl I


Name
Bgl Inew package  Not blocked by 
GC(5mC)NNNNNGGC methylation   
Cat. #E025E026
Package, u.a.5002500
Concentration, u.a./ml5000-100005000-10000

Recognition site
GCCNNNNNGGC
CGGNNNNNCCG
SourceBacillus globigii
Assayed onLambda DNA
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Optimal SE-buffer2W (20 mM Tris-HCl (pH 8,5 at 25°C); 10 mM MgCl2; 200 mM NaCl; 1 mM DTT)
Enzyme activity (%)
BGOWYRose
50 - 7550 - 750 - 1075 - 10025 - 50100
Optimal temperature
37oC
Storage conditions10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
LigationsAfter 10-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C.
Reagents Supplied with Enzyme 10 X SE-buffer 2W
Methylation sensitivityNot blocked by dcm methylation (CmCWGG): GCCWGGNNGGC
Inactivation 20 minutes under
65oC
NotesHigh enzyme concentration may result in star activity
Quality control
Restriction Endonucleases: Quality Control
MSDS:Download MSDS as PDF
References:Duncan, C.H., Wilson, G.A. and Young, F.E. J. Bacteriol. 134: 338 (1978).