Product info: Bpu14 I Name Bpu14 I Cat. # E033 E034 Package, u.a. 1000 5000 Concentration, u.a./ml 10000 10000 Recognition site TT↑CGAA AAGC↓TT Source Bacillus pumilus 14 Assayed on Lambda DNA Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. Optimal SE-buffer G (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.) Enzyme activity (%) B G O W Y Rose 50 - 75 100 25 - 50 25 - 50 75 - 100 100 Optimal temperature 37oC Storage conditions 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol; Store at -20°C. Ligation After 10-fold overdigestion with enzyme about 95% of the DNA fragments can be ligated and recut. Non-specific hydrolisis No nonspecific activity was detected after incubation of 1 ug of Lambda DNA with 20 u.a. of enzyme for 16 hours at 37°C. Reagents Supplied with Enzyme 10 X SE-buffer G Methylation sensitivity not tested Inactivation 20 minutes under 65oC Notes Quality control Restriction Endonucleases: Quality Control MSDS: Download MSDS as PDF References: Semenchenko, G.V., Rechkunova, N.I., Degtyarev, S.Kh. Unpublished observations (1994).