Product info: Bsa29 I Name Bsa29 I      Cat. # E205 E206 Package, u.a. 1000 5000 Concentration, u.a./ml 20000 20000 Recognition site AT↑CGAT TAGC↓TA Source Bacillus stearothermophilus 29 Assayed on Lambda DNA (dam-) Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (dam-) in 1 hour at 37°C in a total reaction volume of 50 μl. Optimal SE-buffer G (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.) + BSA Enzyme activity (%) B G O W Y Rose 25 - 50 100 50 - 75 50 - 75 75 - 100 100 Optimal temperature 37oC Storage conditions 10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Store at -20°C. Ligation After 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut. Non-specific hydrolisis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 37°C. Reagents Supplied with Enzyme 10 X SE-buffer G, BSA (except E205T and E206T). Methylation sensitivity Blocked by overlapping Dam methylation (GmATC): GATCGATC. Blocked by CG methylation. Inactivation 20 minutes under 65oC Notes To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 μg/ml. Do not use BSA for long incubation. Quality control Restriction Endonucleases: Quality Control MSDS: Download MSDS as PDF References: Repin, V.E., Degtyarev, S.K. Unpublished observations.