Product info: Bse8 I

Bse8 I
Cat. #E147E148
Package, u.a.10005000
Concentration, u.a./ml50005000

Recognition site
SourceBacillus species 8
Assayed onLambda DNA
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 60°C in a total reaction volume of 50 μl.
Optimal SE-bufferG (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.)
Enzyme activity (%)
25 - 5010075 - 10075 - 10050 - 75100
Optimal temperature
Storage conditions10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA; 50% glycerol. Store at -20°C.
LigationAfter 5-fold overdigestion with enzyme about 80% of the DNA fragments can be ligated and recut.
Non-specific hydrolisisLong incubation is not recommended. Extended incubations and high concentration of the enzyme may result in star activity.
Reagents Supplied with Enzyme 10 X SE-buffer G
Methylation sensitivity Blocked by overlapping Dam-methylation (GmATC): 5’-GmATCN^NNATC-3’/3’-CTmAGN^NNTAG.
Blocked by overlapping (5mC)-methylation: 5’-GATNN^NNAT(5mC)-3’/3’-(5mC)TANN^NNTAG-5’.
Cuts hemimethylated sequence: 5’-GATNN^NNAT(5mC)-3’/3’-CTANN^NNTAG-5’.

Inactivation 20 minutes under
NotesHigh enzyme concentration (more than 5-fold overdigestion on 1 μg substrate) may result in star activity.
Quality control
Restriction Endonucleases: Quality Control
MSDS:Download MSDS as PDF
References:Repin, V.E., Serov, G.D., Puchkova, L.I., Tereshenko, T.A., Lebedev, L.R., Chigikov, V.E. Bioorg. Khim. 19: 583-585 (1993).