Product info: Bse8 I Name Bse8 I Cat. # E147 E148 Package, u.a. 1000 5000 Concentration, u.a./ml 5000 5000 Recognition site GATNN↑NNATC CTANN↓NNTAG Source Bacillus species 8 Assayed on Lambda DNA Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 60°C in a total reaction volume of 50 μl. Optimal SE-buffer G (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.) Enzyme activity (%) B G O W Y Rose 25 - 50 100 75 - 100 75 - 100 50 - 75 100 Optimal temperature 60oC Storage conditions 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA; 50% glycerol. Store at -20°C. Ligations After 5-fold overdigestion with enzyme about 80% of the DNA fragments can be ligated and recut. Non-specific hydrolisis Long incubation not recommended owing to occurrence of star activity Reagents Supplied with Enzyme 10 X SE-buffer G Methylation sensitivity not tested Inactivation 20 minutes under 80oC Notes High enzyme concentration (more than 5-fold overdigestion on 1 μg substrate) may result in star activity. Quality control Restriction Endonucleases: Quality Control MSDS: Download MSDS as PDF References: Repin, V.E., Serov, G.D., Puchkova, L.I., Tereshenko, T.A., Lebedev, L.R., Chigikov, V.E. Bioorg. Khim. 19: 583-585 (1993).