Recognition site | CC↑TNAGG GGANT↓CC |
Source | Bacillus species 21 |
Assayed on | Lambda DNA (HindIII-digest) |
Unit definition | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (HindIII-digest) in 1 hour at 37°C in a total reaction volume of 50 μl. |
Optimal SE-buffer | Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) |
Enzyme activity (%) | B | G | O | W | Y | Rose | 50 - 75 | 50 - 75 | 10 - 25 | 25 - 50 | 100 | 40 |
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Optimal temperature | 37oC |
Storage conditions | 10 mM KH2PO4 (pH 7.4); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Store at -20°C. |
Ligation | After 2-fold overdigestion with enzyme about 50% of DNA fragments can be ligated by using of high concentration T4 DNA ligase with presence of 10% PEG. Of these more than 90% can be recut. |
Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 37°C.
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Reagents Supplied with Enzyme |
10 X SE-buffer Y |
Methylation sensitivity | not tested |
Inactivation 20 minutes under | 80oC |
Quality control | Restriction Endonucleases: Quality Control |
MSDS: | Download MSDS as PDF |
References: | Degtyarev, S.Kh., Rechkunova, N.I., Repin, V.E., Kolyhalov, A.A., Netesov, S.V. Izv. Sib. Otd. Akad. Nauk SSSR 1: 138-139 (1990).
V.A. Chernukhin, M.A. Abdurashitov, V.N. Tomilov, D.A. Gonchar, S.Kh. Degtyarev Comparative restriction enzymes analysis of rat chromosomal DNA in vitro and in silico // "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.2, No 3, pp 39-46, 2006
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