Product info: Bso31 I


Name
Bso31 Inew package  Not blocked by
  GGTCT(5mC) methylation  
Cat. #E285E286
Package, u.a.2001000
Concentration, u.a./ml5000-100005000-10000

Recognition site
GGTCTC(N)1
CCAGAG(N)5
SourceBacillus stearothermophilus 31
Assayed onT7 DNA
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of T7 DNA in 1 hour at 55°C in a total reaction volume of 50 μl.
Optimal SE-bufferO (50 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) + BSA
Enzyme activity (%)
BGOWYRose
25 - 5075 - 10010075 - 10025 - 5040
Optimal temperature
55oC
Storage conditions10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA; 50% glycerol; Store at -20°C.
LigationsAfter 5-fold overdigestion with enzyme more than 90% of DNA fragments can be ligated. Of these 80% can be recut.
Non-specific hydrolisisNo nonspecific activity was detected after incubation of 1 μg of T7 DNA with 5 u.a. of enzyme for 16 hours at 55°C.
Reagents Supplied with Enzyme 10 X SE-buffer O, BSA
Methylation sensitivityNot blocked by methylation GGTCTmC
Inactivation 20 minutes under
80oC
NotesTo obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 μg/ml.
Do not use BSA for long incubation.
Quality control
Restriction Endonucleases: Quality Control
MSDS:Download MSDS as PDF
References:Shinkarenko, N.M., Lebedeva, N.A., Dedkov, V.S., Abdurashitov, M.A., Degtyarev, S.K. Unpublished observations (1999).
Theoretical diagrams of DNA digestion by this enzyme for the most known DNA substrates:

To view the fragments length values please point mouse cursor over diagram
Fragment lengths
M12345M

M - ladder, 1 - Adeno-2 DNA, 2 - Lambda DNA, 3 - T7 DNA, 4 - pUC19, 5 - pBR322