Name | BstMA I | |
| Cat. # | E291 | E292 | Package, u.a. | 2000 | 10000 | Concentration, u.a./ml | 30000 | 30000 |
Recognition site | GTCTC(N)1↑ CAGAG(N)5↓ | Source | Bacillus stearothermophilus MA | Assayed on | Lambda DNA | Unit definition | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 55°C in a total reaction volume of 50 μl. | Optimal SE-buffer | W (10 mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) + BSA | Enzyme activity (%) | B | G | O | W | Y | Rose | 25 - 50 | 50 - 75 | 50 - 75 | 100 | 75 - 100 | 100 |
| Optimal temperature | 55oC | Storage conditions | 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA; 50% glycerol; Store at -20°C. | Ligation | After 50-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut. | Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 100 u.a. of enzyme for 16 hours at 55°C.
| Reagents Supplied with Enzyme |
10 X SE-buffer W, BSA | Methylation sensitivity | not tested | Inactivation 20 minutes under | 65oC | Notes | To obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 μg/ml.
Do not use BSA for long incubation. | Quality control | Restriction Endonucleases: Quality Control | MSDS: | Download MSDS as PDF | References: | Chernukhin, V.A., Mikhnenkova, N.A., Kileva, E.V., Mezentseva, N.V., Dedkov, V.S., Degtyarev, S.K. Unpublished observations (2005)
Murat A Abdurashitov, Victor N Tomilov, Valery A Chernukhin, S. Kh. Degtyarev A physical map of human Alu repeats cleavage by restriction endonucleases // BMC Genomics 2008, 9:305
Abdurashitov M.A., Tomilov V.N., Chernukhin V.A., Gonchar D. A., Degtyarev S. Kh. Comparative analysis of human chromosomal DNA digestion with restriction endonucleases in vitro and in silico. // "Medical genetics" V.6, No 8, pp 29-36, 2007
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