Product info: Bsp13 I


Name
Bsp13 Inew package  Blocked by TCCGG(6mA)
 and (6ma)TCCGGA methylation  
Cat. #E183E184
Package, u.a.10005000
Concentration, u.a./ml10000-2000010000-20000

Recognition site
TCCGGA
AGGCCT
SourceBacillus species 13
Assayed onLambda DNA (dam-)
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (dam-) in 1 hour at 50°C in a total reaction volume of 50 μl.
Optimal SE-buffer2K (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 200 mM KCl; 1 mM DTT.)
Enzyme activity (%)
BGOWYRose
25 - 5050 - 7575 - 10050 - 750 - 105
Optimal temperature
50oC
Storage conditions10 mM Tris-HCl (pH 7.5); 200 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
LigationsAfter 10-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 50°C.
Reagents Supplied with Enzyme 10 X SE-buffer 2K
Methylation sensitivityBlocked by overlapping Dam methylation (GmATC): TCCGGATC and GATCCGGA.
Inactivation 20 minutes under
65oC
Quality control
Restriction Endonucleases: Quality Control
MSDS:Download MSDS as PDF
References:Dedkov, V.S., Rechkunova, N.I., Degtyarev, S.Kh. Unpublished observations.
Theoretical diagrams of DNA digestion by this enzyme for the most known DNA substrates:

To view the fragments length values please point mouse cursor over diagram
Fragment lengths
M12345M

M - ladder, 1 - Adeno-2 DNA, 2 - Lambda DNA, 3 - T7 DNA, 4 - pUC19, 5 - pBR322