|Source||Bacillus species 13|
|Assayed on||Lambda DNA (dam-)|
|Unit definition||One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (dam-) in 1 hour at 50°C in a total reaction volume of 50 μl.|
|Optimal SE-buffer||2K (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 200 mM KCl; 1 mM DTT.)|
|Enzyme activity (%)|
|25 - 50||50 - 75||75 - 100||50 - 75||0 - 10||5|
|Storage conditions||10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C.|
|Ligation||After 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.|
|Non-specific hydrolisis||No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 50°C.
|Reagents Supplied with Enzyme||
10 X SE-buffer 2K|
|Methylation sensitivity||Blocked by overlapping Dam methylation (GmATC): TCCGGATC and GATCCGGA.|
|Inactivation 20 minutes under||65oC|
|Quality control||Restriction Endonucleases: Quality Control|
|MSDS:||Download MSDS as PDF|
|References:||Dedkov, V.S., Rechkunova, N.I., Degtyarev, S.Kh. Unpublished observations.