Product info: Bsp1720 I


Name
Bsp1720 Inew package  
Cat. #E185E186
Package, u.a.5002500
Concentration, u.a./ml1000010000

Recognition site
GCTNAGC
CGANTCG
SourceBacillus species 1720
Assayed onLambda DNA
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Optimal SE-bufferG (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.)
Enzyme activity (%)
BGOWYRose
50 - 7510050 - 7550 - 7575 - 10075
Optimal temperature
37oC
Storage conditions10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol. Store at -20°C.
LigationsAfter 10-fold overdigestion with enzyme about 80% of DNA fragment can be ligated. Of these about 95% can be recut
Non-specific hydrolisisNo nonspecific activity was detected after incubation of 1μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C
Reagents Supplied with Enzyme 10 X SE-buffer G
Methylation sensitivitynot tested
Inactivation 20 minutes under
80oC
Quality control
Restriction Endonucleases: Quality Control
MSDS:Download MSDS as PDF
References:Degtyarev, S.Kh., Kolyhalov, A.A., Rechkunova, N.T., Tepavicharova, I.I., Mechandjiska, L.I., Builieva, E.I. Nucleic Acids Res. 19: 2504 (1991).