| Recognition site | GC↑TNAGC
CGANT↓CG |
| Source | Bacillus species 1720 |
| Assayed on | Lambda DNA |
| Unit definition | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. |
| Optimal SE-buffer | G (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.) |
| Enzyme activity (%) | | B | G | O | W | Y | Rose | | 50 - 75 | 100 | 50 - 75 | 50 - 75 | 75 - 100 | 75 |
|
| Optimal temperature | 37oC |
| Storage conditions | 10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol. Store at -20°C. |
| Ligation | After 10-fold overdigestion with enzyme about 80% of DNA fragment can be ligated. Of these about 95% can be recut |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C
|
| Reagents Supplied with Enzyme |
10 X SE-buffer G |
| Methylation sensitivity | not tested |
| Inactivation 20 minutes under | 80oC |
| Quality control | Restriction Endonucleases: Quality Control |
| MSDS: | Download MSDS as PDF |
| References: | Degtyarev, S.Kh., Kolyhalov, A.A., Rechkunova, N.T., Tepavicharova, I.I., Mechandjiska, L.I., Builieva, E.I.
Nucleic Acids Res. 19: 2504 (1991).
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