Product info: BssEC I


Name
BssEC I  
Cat. #E273E274
Package, u.a.2001000
Concentration, u.a./ml1000010000

Recognition site
CCNNGG
GGNNCC
SourceBacillus stearothermophilus EC
Assayed onLambda DNA
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 60°C in a total reaction volume of 50 μl.
Optimal SE-bufferY (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)
Enzyme activity (%)
BGOWYRose
50 - 7550 - 7550 - 7575 - 100100100
Optimal temperature
60oC
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
LigationsAfter 10-fold overdigestion with enzyme 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 60°C.
Reagents Supplied with Enzyme 10 X SE-buffer Y
Methylation sensitivitynot tested
Inactivation 20 minutes under
80oC
Quality control
Restriction Endonucleases: Quality Control
MSDS:Download MSDS as PDF
References:Abdurashitov, M.A., Dedkov, V.S., Kileva, E.V., Shevchenko, A.V., Degtyarev, S.K. Unpublished observations (1998).
 Murat A Abdurashitov, Victor N Tomilov, Valery A Chernukhin, S. Kh. Degtyarev A physical map of human Alu repeats cleavage by restriction endonucleases // BMC Genomics 2008, 9:305
 Abdurashitov M.A., Tomilov V.N., Chernukhin V.A., Gonchar D. A., Degtyarev S. Kh. Comparative analysis of human chromosomal DNA digestion with restriction endonucleases in vitro and in silico. // "Medical genetics" V.6, No 8, pp 29-36, 2007