Product info: BssT1 I


Name
BssT1 I
Cat. #E207E208
Package, u.a.10005000
Concentration, u.a./ml2000020000

Recognition site
CCWWGG
GGWWCC
SourceBacillus stearothermophilus T1
Assayed onLambda DNA
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 60°C in a total reaction volume of 50 μl.
Optimal SE-buffer2K (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 200 mM KCl; 1 mM DTT.)
Enzyme activity (%)
BGOWYRose
10 - 2525 - 5025 - 5075 - 10010 - 25100
Optimal temperature
60oC
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
LigationsAfter 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 60°C.
Reagents Supplied with Enzyme 10 X SE-buffer 2K
Methylation sensitivitynot tested
Inactivation 20 minutes under
80oC
NotesHigh enzyme concentration may result in star activity
Quality control
Restriction Endonucleases: Quality Control
MSDS:Download MSDS as PDF
References:Serov, G.D., Tereshenko, T.A., Puchkova, L.I., Rechkunova N.I., Degtyarev, S.K., Unpublished observations.
Theoretical diagrams of DNA digestion by this enzyme for the most known DNA substrates:

To view the fragments length values please point mouse cursor over diagram
Fragment lengths
M12345M

M - ladder, 1 - Adeno-2 DNA, 2 - Lambda DNA, 3 - T7 DNA, 4 - pUC19, 5 - pBR322