Product info: Acc65 I


Name
Acc65 IBlocked by GGTAC(5mC) methylation  
Cat. #E003E004E003TE004TE003m
Package, u.a.1000500010005000500
Concentration, u.a./ml10000-3000010000-3000010000-3000010000-3000010000

Recognition site
GGTACC
CCATGG
SourceAcinetobacter calcoaceticus 65
Assayed onLambda DNA(dcm-)
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA(dcm-) in 1 hour at 37°C in a total reaction volume of 50 μl.
Optimal SE-bufferW (10 mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.)
Enzyme activity (%)
BGOWYRose
10 - 2525 - 5075 - 10010010 - 25100
Optimal temperature
37oC
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
LigationsAfter 10-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C.
Reagents Supplied with Enzyme 10 X SE-buffer W
For E003T and E004T an additional 10X SE-buffer ROSE is supplied.
Methylation sensitivityBlocked by overlapping Dcm methylation(CmCWGG): GGTACCWGG.
Inactivation 20 minutes under
65oC
Quality control
Restriction Endonucleases: Quality Control
MSDS:Download MSDS as PDF
References:Prichodko, G.G., Rechkunova, N.I., Repin, V.E., Degtyarev, S.Kh., Sib. Biol. J. 1: 59-60 (1991).
Theoretical diagrams of DNA digestion by this enzyme for the most known DNA substrates:

To view the fragments length values please point mouse cursor over diagram
Fragment lengths
M12345M

M - ladder, 1 - Adeno-2 DNA, 2 - Lambda DNA, 3 - T7 DNA, 4 - pUC19, 5 - pBR322