Product info: Polynucleotide Kinase T4


Name
Polynucleotide Kinase T4
Cat. #E311E312
Package, u.a.5002500
Concentration, u.a./ml1000010000

SourceIsolated from E.coli strain that carries the cloned Polynucleotide kinase gene from bacteriophage T4
DescriptionT4 polynucleotide Kinase catalyzes the transfer and exchange of Pi from the γ position of ATP to the 5’ hydroxyl terminus of polynucleotides (double- and single-stranded DNA and RNA) and nucleoside 3’-monophosphates. The enzyme also catalyzes the removal of 3’ - phosphoryl groups from 3’ - phosphoril polynucleotides, deoxynucleoside 3’ - monophosphates and deoxynucleoside 3’ - diphosphates.
Applications:
- end-labeling DNA or RNA for probes and DNA sequencing;
- addition of 5’ - phosphates to oligonucleotides to allow subsequent ligation;
- removal of 3’ - phosphoril groups.
Unit definitionOne unit is the amount of enzyme catalyzing the incorporation of 1 nmol of acid-insoluble [32P] in 30 minutes at 37°C.
Unit Assay Conditions: 1 x T4 polynucleotide kinase Buffer, 66 µM [γ- 32P] ATP (5 x 106 cpm/µmol) and 0.26 mM 5’ - hydroxyl - terminated salmon sperm DNA.
Reaction bufferSE-buffer polynucleotide kinase T4, (50 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 5 mM DTT.)
Optimal temperature
37oC
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM NaCl; 0.1 mM EDTA; 1 mM DTT; 50% glycerol; Store at -20°C.
Quality controlThe enzyme is purified free of contaminating endonucleases, exonucleases, phosphatases.
MSDS:Download MSDS as PDF