Product info: DNA polymerase I E.coli (Klenow Fragment)


Name
DNA polymerase I E.coli (Klenow Fragment)
Cat. #E325E326
Package, u.a.2001000
Concentration, u.a./ml50005000

SourceIsolated from E.coli strain that carries the cloned DNA Polymerase I, Large (Klenow) Fragment gene.
DescriptionDNA Polymerase I, Large (Klenow) Fragment is a proteolytic product of E. coli DNA Polymerase I which retains polymerization and 3` -> 5` exonuclease activity, but has lost 5` -> 3` exonuclease activity.
Applications:
- dideoxy sequencing;
- polishing ends;
- second strand cDNA synthesis
Unit definitionOne unit is the amount of enzyme required to incorporate 10 nmol of dNTPs into an acid-insoluble material in 30 minutes at 37°C.
Unit Assay Conditions: 1 x Klenow Buffer, 33 μM dNTP including [3H]-dTTP and 70 μg/ml denatured calf thymus DNA.
Reaction bufferSE-buffer Klenow fragment, (50 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 5 mM DTT.)
Optimal temperature
37oC
Storage conditions10 mM KH2PO4 (pH 7.5); 50 mM KCl; 0.5 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol. Store at -20°C.
Quality controlThe enzyme is purified free of contaminating endonucleases and exonucleases.
MSDS:Download MSDS as PDF