|Source||Isolated from E.coli strain that carries the cloned T4 DNA polymerase gene|
|Description||T4 DNA Polymerase catalyzes the synthesis of DNA in the 5` -> 3` direction and requires the presence of template and primer. This enzyme has a 3` -> 5` exonuclease activity.|
- polishing ends;
- probe labeling using replacement synthesis.
|Unit definition||One unit is the amount of enzyme required to incorporate 10 nmol of dNTP into acid precipitable material in 30 minutes at 37°C.
Unit Assay Conditions: 1 x T4 DNA Polymerase Reaction Buffer, 33 μM dNTPs including [3H]-dTTP and 70 μg/ml denatured calf thymus DNA.
|Reaction buffer||SE-buffer DNA polymerase T4, (67 mM Tris-HCl (pH 8.8 at 25°C); 6.7 mM MgCl2;|
16.7 mM (NH4)2SO4, 1 mM DTT.)
|Storage conditions||20 mM Tris-HCl (pH 7.5); 50 mM KCl; 10 mM 2-mercaptoethanol ; 50% glycerol. Store at -20°C.|
|Quality control||The enzyme is purified free of contaminating endonucleases and
|MSDS:||Download MSDS as PDF|