Product info: T4 DNA polymerase


Name
T4 DNA polymerase
Cat. #E339E340
Package, u.a.2001000
Concentration, u.a./ml2000-50002000-5000

SourceIsolated from E.coli strain that carries the cloned T4 DNA polymerase gene
DescriptionT4 DNA Polymerase catalyzes the synthesis of DNA in the 5` -> 3` direction and requires the presence of template and primer. This enzyme has a 3` -> 5` exonuclease activity.
Applications:
- polishing ends;
- probe labeling using replacement synthesis.
Unit definitionOne unit is the amount of enzyme required to incorporate 10 nmol of dNTP into acid precipitable material in 30 minutes at 37°C. Unit Assay Conditions: 1 x T4 DNA Polymerase Reaction Buffer, 33 μM dNTPs including [3H]-dTTP and 70 μg/ml denatured calf thymus DNA.
Reaction bufferSE-buffer DNA polymerase T4, (67 mM Tris-HCl (pH 8.8 at 25°C); 6.7 mM MgCl2;
16.7 mM (NH4)2SO4, 1 mM DTT.)
Optimal temperature
37oC
Storage conditions20 mM Tris-HCl (pH 7.5); 50 mM KCl; 10 mM 2-mercaptoethanol ; 50% glycerol. Store at -20°C.
Quality controlThe enzyme is purified free of contaminating endonucleases and exonucleases.
MSDS:Download MSDS as PDF