Product info: T4 DNA polymerase


Name
T4 DNA polymerase
Cat. #E339E340
Package, u.a.2001000
Concentration, u.a./ml2000-50002000-5000

SourceIsolated from E.coli strain that carries the cloned T4 DNA polymerase gene
DescriptionT4 DNA Polymerase catalyzes the synthesis of DNA in the 5 -> 3 direction and requires the presence of template and primer. This enzyme has a 3 -> 5 exonuclease activity.
Applications:
- polishing ends;
- probe labeling using replacement synthesis.
Unit definitionOne unit is the amount of enzyme required to incorporate 10 nmol of dNTP into acid precipitable material in 30 minutes at 37C. Unit Assay Conditions: 1 x T4 DNA Polymerase Reaction Buffer, 33 M dNTPs including [3H]-dTTP and 70 g/ml denatured calf thymus DNA.
Reaction bufferSE-buffer DNA polymerase T4, (67 mM Tris-HCl (pH 8.8 at 25°C); 6.7 mM MgCl2;
16.7 mM (NH4)2SO4, 1 mM DTT.)
Optimal temperature
37oC
Storage conditions20 mM Tris-HCl (pH 7.5); 50 mM KCl; 10 mM 2-mercaptoethanol ; 50% glycerol. Store at -20C.
Quality controlThe enzyme is purified free of contaminating endonucleases and exonucleases.
MSDS:Download MSDS as PDF