| Source | Isolated from E.coli strain that carries the cloned Reverse transcriptase gene |
| Description | Moloney Murine Leukemia Virus (M-MuLV) Reverse Transcriptase is an RNA directed DNA polymerase. This enzyme can synthesize a complementary DNA strand initiating from a primer using either RNA (cDNA synthesis) or single-stranded DNA as a template. The absence of RNase H activity enhances the synthesis of long cDNAs and therefore the enzyme is recommended for preparing long cDNAs.
Application: first strand cDNA synthesis.
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| Unit definition | One unit is the amount of the enzyme required to incorporate 1 nmol of dTTP into an acid insoluble material in 10 minutes at 37°C using poly(rA)•oligo(dT).
Unit Assay Conditions: 50 mM Tris-HCl (pH 8.3), 6 mM MgCl2, 10 mM DTT, 0.5 mM [3H]-dTTP, 0.4 mM poly(rA)•oligo(dT) 12-18.
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| Reaction buffer | SE-buffer Reverse transcriptase M-MulV, (50 mM Tris-HCl (pH 8.3 at 25°C); 3 mM MgCl2; 75 mM KCl; 10 mM DTT.) |
| Optimal temperature | 37oC |
| Storage conditions | 10 mM KH2PO4 (pH 7.5); 0,1 mM EDTA; 200 mM NaCl; 7 mM 2-mercaptoethanol; 50% glycerol. Store at -20°C. |
| Notes | High enzyme concentration may lead to RT-PCR inhibition. In this case the enzyme preparation should be diluted in 5, 10 or 20 times with M-MuLV Reverse Transcriptase dilution Buffer (supplemented) |
| Quality control | The enzyme is purified free of contaminating endonucleases and
exonucleases.
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| MSDS: | Download MSDS as PDF |