Product info: Uracil DNA Glycosylase


Name
Uracil DNA Glycosylasenew package  
Cat. #E335E336
Package, u.a.10005000
Concentration, u.a./ml20000-5000020000-50000

SourceIsolated from the recombinant source
DescriptionE.coli uracil-DNA glycosylase (UDG) catalyzes the release of free uracil from uracil-containing DNA. UDG efficiently hydrolyzes uracil from single-stranded or double-stranded DNA, but not from oligomers (6 or fewer bases)
Applications:
- site-directed mutagenesis;
- as a probe for protein-DNA interaction studies;
Treatment of 1 g of uracil-containing DNA with 1 unit of UDG for 10 minutes at 37C renders the DNA incapable of being copied by DNA polymerase. The enzyme can be 95% heat killed by incubation at 95C for 10 minutes.
Unit definitionOne unit is defined as the amount of enzyme that catalyzes the release of 60 pmol of uracil per minute from double-stranded, uracil-containing DNA. Activity is measured by release of [3H]-uracil in a 50 l reaction containing 0,2 g DNA (104 105 cpm/g) in 30 minutes at 37C.
Reaction bufferSE-buffer Uracil DNA Glycosylase, (20 mM Tris-HCl (pH 8.0 at 25°C); 1 mM EDTA; 1 mM DTT)
Optimal temperature
37oC
Storage conditions10 mM Tris-HCl (pH 7.6); 50 mM NaCl; 1 mM EDTA; 1 mM DTT; 50% glycerol. Store at -20C.
MSDS:Download MSDS as PDF