Product info: Uracil DNA Glycosylase Name Uracil DNA Glycosylase    Cat. # E335 E336 Package, u.a. 1000 5000 Concentration, u.a./ml 20000-50000 20000-50000 Source Isolated from the recombinant source Description E.coli uracil-DNA glycosylase (UDG) catalyzes the release of free uracil from uracil-containing DNA. UDG efficiently hydrolyzes uracil from single-stranded or double-stranded DNA, but not from oligomers (6 or fewer bases) Applications: - site-directed mutagenesis; - as a probe for protein-DNA interaction studies; Treatment of 1 µg of uracil-containing DNA with 1 unit of UDG for 10 minutes at 37°C renders the DNA incapable of being copied by DNA polymerase. The enzyme can be 95% heat killed by incubation at 95°C for 10 minutes. Unit definition One unit is defined as the amount of enzyme that catalyzes the release of 60 pmol of uracil per minute from double-stranded, uracil-containing DNA. Activity is measured by release of [3H]-uracil in a 50 µl reaction containing 0,2 µg DNA (104 – 105 cpm/µg) in 30 minutes at 37°C. Reaction buffer SE-buffer Uracil DNA Glycosylase, (20 mM Tris-HCl (pH 8.0 at 25°C); 1 mM EDTA; 1 mM DTT) Optimal temperature 37oC Storage conditions 10 mM Tris-HCl (pH 7.6); 50 mM NaCl; 1 mM EDTA; 1 mM DTT; 50% glycerol. Store at -20°C. MSDS: Download MSDS as PDF