Product info: Alkaline Phosphatase


Name
Alkaline Phosphatase
Cat. #E327E328
Package, u.a.100500
Concentration, u.a./ml1000010000

SourceCalf intestinal mucosa
DescriptionAlkaline phosphatase catalyzes the removal of 5' phosphate groups from DNA, RNA and ribo- and deoxyribonucleoside triphosphates.
Applications:
- removing 5` and 3` phosphoryl groups from nucleic acids;
- preparing templates for 5` end labeling;
- preventing fragments from self ligating;
- dephosphorylation of proteins
Unit definitionOne unit is the amount of enzyme that hydrolyzes 1 mol of p-nitrophenylphosphate to p- nitrophenol in a total reaction volume of 1 ml in 1 min at 37C.
Unit Assay Conditions: 1 M diethanolamine-HCl (pH 9.8), 0.5 mM MgCl2 and 10 mM p-nitrophenylphosphate. These conditions are only used for quantitating enzyme activity.
Reaction bufferSE-buffer O, (50 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.)
Optimal temperature
37oC
Storage conditions50 mM KCl, 10 mM Tris-HCl (pH 8.2), 1 mM MgCl2, 0.1 mM ZnCl2, 50% glycerol. Store at -20C
Quality controlThe enzyme is purified free of contaminating exonuclease, endonuclease, ribonuclease activities.
MSDS:Download MSDS as PDF