|Product info: Exonuclease III|
|Exonuclease III|| |
|Cat. #||E345||E346 |
|Package, u.a.||5000||25000 |
|Concentration, u.a./ml||40000-100000||40000-100000 |
|Source||Isolated from E.coli.|
|Description||Description: Exonuclease III (E. coli) catalyzes the stepwise removal of mononucleotides from 3`- hydroxyl termini of double stranded DNA. Exonuclease III activity depends partially on helical structure and displays sequence dependence (C > A = T > G). Temperature, salt concentration and the ratio of enzyme to DNA greatly affect enzyme activity, requiring reaction conditions to be tailored to specific applications.|
- unidirectional nested deletions;
- site-directed mutagenesis;
- preparation of strand-specific probes;
- preparation of single-stranded substrates for dideoxy sequencing.
|Unit definition||One unit is the amount of enzyme required to produce 1 nmol of acid soluble total nucleotide in 30 minutes at 37°C.|
Unit Assay Conditions: 50 mM Tris-HCl (pH 7.5 at 25°C); 1 mM MgCl2; 1 mM DTT, 0.15 mM sonicated pancreatic DNA.
|Reaction buffer||SE-buffer Exonuclease III, (50 mM Tris-HCl (pH 7.6 at 25°C); 1 mM MgCl2.)|
|Storage conditions||10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.5 mM EDTA; 1 mM 2-mercaptoethanol; 50% glycerol. Store at -20°C.|
|MSDS:||Download MSDS as PDF|