Product info: RNA ligase T4


Name
RNA ligase T4
Cat. #E349E350
Package, u.a.10005000
Concentration, u.a./ml1000010000

SourceIsolated from E.coli strain that carries the cloned RNA ligase gene from bacteriophage T4
DescriptionRNA Ligase catalyzes ligation of a 5 phosphoryl - terminated nucleic acid donor to a 3 hydroxyl - terminated nucleic acid acceptor through the formation of a 3 -> 5 phosphodiester bond, with hydrolysis of ATP to AMP and PPi. Substrates include single-stranded RNA and DNA as well as dinucleoside pyrophosphates.
Applications:
- labeling of 3-termini of RNA with 5 - [32P] pCp;
- inter- and intra-molecular joining of RNA and DNA molecules.
Unit definitionOne unit is defined as the amoumt of enzyme required to convert of 1 pmol of [3H]ATP in AMP ligase complex in 15 minutes at 25C.
Unit Assay Conditions: 50 mM Tris-HCl (pH 7.5), 10 mM MgCl2, 10 mM DTT, 0.2 M [3H]ATP.
Reaction bufferSE-buffer RNA ligase T4, (50 mM Tris-HCl (pH 7.8 at 25°C); 10 mM MgCl2; 10 mM DTT; 1 mM ATP.)
Optimal temperature
37oC
Storage conditions10 mM Tris-HCl (pH 7.4); 50 mM KCl; 0.1 mM EDTA; 1 mM DTT; 50% glycerol. Store at -20C.
Quality controlThe enzyme is purified free of contaminating endonucleases and exonucleases.
MSDS:Download MSDS as PDF