|Source||Isolated from E.coli strain that carries the cloned RNA ligase gene from bacteriophage T4|
|Description||RNA Ligase catalyzes ligation of a 5’ phosphoryl - terminated nucleic acid donor to a 3’ hydroxyl - terminated nucleic acid acceptor through the formation of a 3’ -> 5’ phosphodiester bond, with hydrolysis of ATP to AMP and PPi. Substrates include single-stranded RNA and DNA as well as dinucleoside pyrophosphates. |
- labeling of 3’-termini of RNA with 5’ - [32P] pCp;
- inter- and intra-molecular joining of RNA and DNA molecules.
|Unit definition||One unit is defined as the amoumt of enzyme required to convert of 1 pmol of [3H]ATP in AMP –ligase complex in 15 minutes at 25°C.|
Unit Assay Conditions: 50 mM Tris-HCl (pH 7.5), 10 mM MgCl2, 10 mM DTT, 0.2 µM [3H]ATP.
|Reaction buffer||SE-buffer RNA ligase T4, (50 mM Tris-HCl (pH 7.8 at 25°C); 10 mM MgCl2; 10 mM DTT; 1 mM ATP.)|
|Storage conditions||10 mM Tris-HCl (pH 7.4); 50 mM KCl; 0.1 mM EDTA; 1 mM DTT; 50% glycerol. Store at -20°C.|
|Quality control||The enzyme is purified free of contaminating endonucleases and
|MSDS:||Download MSDS as PDF|