Product info: Tli Inorganic Pyrophosphatase


Name
Tli Inorganic Pyrophosphatase
Cat. #E315E316
Package, u.a.100500
Concentration, u.a./ml10001000

SourceIsolated from E.coli strain that carries the cloned Inorganic pyrophosphatase gene from Thermococcus litoralis.
DescriptionTli-Inorganic Pyrophosphatase catalyzes the hydrolysis of inorganic pyrophosphate to form orthophosphate: P2O7-4+H2O -> 2HPO4-2. Enzyme is extremely thermostable.
Unit definitionOne unit is the amount of enzyme that will generate 40 nmoles of phosphate per minute from pyrophosphate under standard reaction conditions (a 10 minute reaction at 75C in 50 mM Tris-HCl (pH 8.5), 1 mM MgCl2, 0.32 mM PPi, reaction volume of 0.5 ml).
Reaction bufferSE-buffer Inorganic pyrophosphatase, (50 mM Tris-HCl (pH 8.5 at 25°C); 1 mM MgCl2.)
Optimal temperature
75oC
Storage conditions20 mM Tris-HCl (pH 7.6); 0.1 mM EDTA; 100 mM KCl, 0.2% Tween-20; 50% glycerol. Store at -20C.
Quality controlThe enzyme is purified free of contaminating endonucleases and exonucleases.
MSDS:Download MSDS as PDF