Product info: BstBA I


Name
BstBA IBlocked by 
YA(5mC)GTR methylation  
Cat. #E237E238
Package, u.a.5002500
Concentration, u.a./ml5000-200005000-20000

Recognition site
YACGTR
RTGCAY
SourceBacillus stearothermophilus BA
Assayed onLambda DNA
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 65°C in a total reaction volume of 50 μl.
Optimal SE-bufferW (10 mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) + BSA
Enzyme activity (%)
BGOWYRose
25 - 5025 - 5075 - 10010025 - 5050
Optimal temperature
65oC
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 10 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
LigationsAfter 10-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 65°C.
Reagents Supplied with Enzyme 10 X SE-buffer W, BSA
Methylation sensitivityBlocked by CG methylation.
Inactivation 20 minutes under
80oC
NotesTo obtain 100% activity, BSA should be added to the 1x reaction mix to a final concentration of 100 μg/ml.
Do not use BSA for long incubation.
Quality control
Restriction Endonucleases: Quality Control
MSDS:Download MSDS as PDF
References:Abdurashitov, M.A., Shinkarenko, N.M., Dedkov, V.S., Shevchenko, A.V., Degtyarev, S.K. Unpublished observations (1996).
Theoretical diagrams of DNA digestion by this enzyme for the most known DNA substrates:

To view the fragments length values please point mouse cursor over diagram
Fragment lengths
M12345M

M - ladder, 1 - Adeno-2 DNA, 2 - Lambda DNA, 3 - T7 DNA, 4 - pUC19, 5 - pBR322