Name | BstDE I | |
| Cat. # | E227 | E228 | Package, u.a. | 1000 | 5000 | Concentration, u.a./ml | 20000 | 20000 |
Recognition site | C↑TNAG GANT↓C | Source | Bacillus stearothermophilus DE | Assayed on | Lambda DNA | Unit definition | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 60°C in a total reaction volume of 50 μl. | Optimal SE-buffer | G (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.) | Enzyme activity (%) | B | G | O | W | Y | Rose | 75 - 100 | 100 | 25 - 50 | 50 - 75 | 10 - 25 | 100 |
| Optimal temperature | 60oC | Storage conditions | 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol; Store at -20°C. | Ligation | After 20-fold overdigestion with enzyme 90% of the DNA fragments can be ligated and recut | Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 40 u.a. of enzyme for 16 hours at 60°C.
| Reagents Supplied with Enzyme |
10 X SE-buffer G
| Methylation sensitivity | not tested | Inactivation 20 minutes under | 80oC | Notes | At 37°C activity is 50%-75% from maximum.
| Quality control | Restriction Endonucleases: Quality Control | MSDS: | Download MSDS as PDF | References: | Shinkarenko, N.M., Shevchenko, A.V., Dedkov, V.S., Abdurashitov, M.A., Degtyarev, S.K. Unpublished observations (1995).
Murat A Abdurashitov, Victor N Tomilov, Valery A Chernukhin, S. Kh. Degtyarev A physical map of human Alu repeats cleavage by restriction endonucleases // BMC Genomics 2008, 9:305
Abdurashitov M.A., Tomilov V.N., Chernukhin V.A., Gonchar D. A., Degtyarev S. Kh. Comparative analysis of human chromosomal DNA digestion with restriction endonucleases in vitro and in silico. // "Medical genetics" V.6, No 8, pp 29-36, 2007
V.A. Chernukhin, M.A. Abdurashitov, V.N. Tomilov, D.A. Gonchar, S.Kh. Degtyarev Comparative restriction enzymes analysis of rat chromosomal DNA in vitro and in silico // "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.2, No 3, pp 39-46, 2006
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