Product info: BstDE I Name BstDE I      Cat. # E227 E228 Package, u.a. 1000 5000 Concentration, u.a./ml 20000 20000 Recognition site C↑TNAG GANT↓C Source Bacillus stearothermophilus DE Assayed on Lambda DNA Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 60°C in a total reaction volume of 50 μl. Optimal SE-buffer G (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.) Enzyme activity (%) B G O W Y Rose 75 - 100 100 25 - 50 50 - 75 10 - 25 100 Optimal temperature 60oC Storage conditions 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol; Store at -20°C. Ligations After 30-fold overdigestion with enzyme 90% of the DNA fragments can be ligated and recut Non-specific hydrolisis No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 60 u.a. of enzyme for 16 hours at 60°C. Reagents Supplied with Enzyme 10 X SE-buffer G Methylation sensitivity not tested Inactivation 20 minutes under 80oC Notes At 37°C activity is 10% from maximum Quality control Restriction Endonucleases: Quality Control MSDS: Download MSDS as PDF References: Shinkarenko, N.M., Shevchenko, A.V., Dedkov, V.S., Abdurashitov, M.A., Degtyarev, S.K. Unpublished observations (1995).  Murat A Abdurashitov, Victor N Tomilov, Valery A Chernukhin, S. Kh. Degtyarev A physical map of human Alu repeats cleavage by restriction endonucleases // BMC Genomics 2008, 9:305  Abdurashitov M.A., Tomilov V.N., Chernukhin V.A., Gonchar D. A., Degtyarev S. Kh. Comparative analysis of human chromosomal DNA digestion with restriction endonucleases in vitro and in silico. // "Medical genetics" V.6, No 8, pp 29-36, 2007  V.A. Chernukhin, M.A. Abdurashitov, V.N. Tomilov, D.A. Gonchar, S.Kh. Degtyarev Comparative restriction enzymes analysis of rat chromosomal DNA in vitro and in silico // "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.2, No 3, pp 39-46, 2006