Product info: BstDE I


Name
BstDE I    
Cat. #E227E228
Package, u.a.5002500
Concentration, u.a./ml10000-2000010000-20000

Recognition site
CTNAG
GANTC
SourceBacillus stearothermophilus DE
Assayed onLambda DNA
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 60°C in a total reaction volume of 50 μl.
Optimal SE-bufferG (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.)
Enzyme activity (%)
BGOWYRose
75 - 10010025 - 5050 - 7510 - 25100
Optimal temperature
60oC
Storage conditions10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol; Store at -20°C.
LigationsAfter 30-fold overdigestion with enzyme 90% of the DNA fragments can be ligated and recut
Non-specific hydrolisisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 60 u.a. of enzyme for 16 hours at 60°C.
Reagents Supplied with Enzyme 10 X SE-buffer G
Methylation sensitivitynot tested
Inactivation 20 minutes under
80oC
NotesAt 37°C activity is 10% from maximum
Quality control
Restriction Endonucleases: Quality Control
MSDS:Download MSDS as PDF
References:Shinkarenko, N.M., Shevchenko, A.V., Dedkov, V.S., Abdurashitov, M.A., Degtyarev, S.K. Unpublished observations (1995).
 Murat A Abdurashitov, Victor N Tomilov, Valery A Chernukhin, S. Kh. Degtyarev A physical map of human Alu repeats cleavage by restriction endonucleases // BMC Genomics 2008, 9:305
 Abdurashitov M.A., Tomilov V.N., Chernukhin V.A., Gonchar D. A., Degtyarev S. Kh. Comparative analysis of human chromosomal DNA digestion with restriction endonucleases in vitro and in silico. // "Medical genetics" V.6, No 8, pp 29-36, 2007
 V.A. Chernukhin, M.A. Abdurashitov, V.N. Tomilov, D.A. Gonchar, S.Kh. Degtyarev Comparative restriction enzymes analysis of rat chromosomal DNA in vitro and in silico // "Ovchinnikov bulletin of biotechnology and physical and chemical biology" V.2, No 3, pp 39-46, 2006