Product info: BstH2 I


Name
BstH2 I
Cat. #E171E172
Package, u.a.5002500
Concentration, u.a./ml10000-3000010000-30000

Recognition site
RGCGCY
YCGCGR
SourceBacillus stearothermophilus H2
Assayed onLambda DNA
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 65°C in a total reaction volume of 50 μl.
Optimal SE-bufferY (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) + BSA
Enzyme activity (%)
BGOWYRose
50 - 7550 - 750 - 1010 - 25100100
Optimal temperature
65oC
Storage conditions10 mM KH2PO4(pH 7.2); 100 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Store at -20°C.
LigationsAfter 20-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 65°C.
Reagents Supplied with Enzyme 10 X SE-buffer Y, BSA
Methylation sensitivitynot tested
Inactivation 20 minutes under
80oC
NotesHigh enzyme concentration may result in star activity
To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of 100 μg/ml.
Do not use BSA for long incubation.
Quality control
Restriction Endonucleases: Quality Control
MSDS:Download MSDS as PDF
References:Abdurashitov, M.A., Kileva, E.V., Shevchenko, A.V., Degtyarev, S.K. Unpublished observations (1994).
Theoretical diagrams of DNA digestion by this enzyme for the most known DNA substrates:

To view the fragments length values please point mouse cursor over diagram
Fragment lengths
M12345M

M - ladder, 1 - Adeno-2 DNA, 2 - Lambda DNA, 3 - T7 DNA, 4 - pUC19, 5 - pBR322