Recognition site | CGRY↑CG GC↓YRGC |
Source | Bacillus stearothermophilus MC |
Assayed on | Lambda DNA |
Unit definition | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 50°C in a total reaction volume of 50 μl. |
Optimal SE-buffer | B (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 1 mM DTT.) + BSA |
Enzyme activity (%) | B | G | O | W | Y | Rose | 100 | 75 - 100 | 10 - 25 | 10 - 25 | 50 - 75 | 100 |
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Optimal temperature | 50oC |
Storage conditions | 10 mM Tris-HCl(pH 7.5); 200 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol. Store at -20°C. |
Ligation | After 5-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut. |
Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 50°C.
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Reagents Supplied with Enzyme |
10 X SE-buffer B, BSA
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Methylation sensitivity | not tested |
Inactivation 20 minutes under | 80oC |
Notes | To obtain 100% activity, BSA should be added to the 1 x reaction mix to a final concentration of
100 μg/ml
Do not use BSA for long incubation.
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Quality control | Restriction Endonucleases: Quality Control |
MSDS: | Download MSDS as PDF |
References: | Belichenko, O.A., Shevchenko, A.V., Dedkov, V.S., Abdurashitov, M.A., Degtyarev, S.K. Unpublished observations (1995).
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