Product info: BstMB I


Name
BstMB IBlocked by 
 G(6mA)TC methylation  Not blocked by 
GAT(5mC) methylation  
Cat. #E119E120
Package, u.a.2001000
Concentration, u.a./ml5000-100005000-10000

Recognition site
GATC
CTAG
SourceBacillus stearothermophillus MB
Assayed onLambda DNA (dam-)
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (dam-) in 1 hour at 65°C in a total reaction volume of 50 μl.
Optimal SE-bufferO (50 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.)
Enzyme activity (%)
BGOWYRose
10 - 2525 - 5010075 - 10010 - 25100
Optimal temperature
65oC
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 200 μg/ml BSA. Store at -20°C.
LigationsAfter 5-fold overdigestion with enzyme more than 95% of the DNA fragments can be ligated and recut
Non-specific hydrolisisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 20 u.a. of enzyme for 16 hours at 65°C
Reagents Supplied with Enzyme 10 X SE-buffer O
Methylation sensitivityBlocked by overlapping Dam methylation(GmATC): GATC .
Not blocked by CG methylation.
Not cut hemimethylated site: 5`- G(6mA)TC-3` / 3`-CTAG-5`
Inactivation 20 minutes under
80oC
Quality control
Restriction Endonucleases: Quality Control
MSDS:Download MSDS as PDF
References:Kovalenko, K., Kileva, E.V., Mezentseva, N.V., Mikhnenkova, N.A., Dedkov, V.S., Degtyarev, S.K. Unpublished observations. (2004)
Theoretical diagrams of DNA digestion by this enzyme for the most known DNA substrates:

To view the fragments length values please point mouse cursor over diagram
Fragment lengths
M12345M

M - ladder, 1 - Adeno-2 DNA, 2 - Lambda DNA, 3 - T7 DNA, 4 - pUC19, 5 - pBR322