Product info: PalA I


Name
PalA IBlocked by 
GG(5mC)G(5mC)GCC methylation  
Cat. #E483E484
Package, u.a.100500
Concentration, u.a./ml500-2000500-2000

Recognition site
GGCGCGCC
CCGCGCGG
SourcePseudomanas alcaligenes BS17
Assayed onLambda DNA
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Optimal SE-bufferY (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)
Enzyme activity (%)
BGOWYRose
25 - 5010 - 250 - 100 - 1010040
Optimal temperature
37oC
Storage conditions10 mM Tris-HCl (pH 7.5); 100 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
LigationsAfter 2-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 2 u.a. of enzyme for 16 hours at 37°C.
Reagents Supplied with Enzyme 10 X SE-buffer Y
Methylation sensitivityBlocked by CG methylation.
Inactivation 20 minutes under
65oC
Quality control
Restriction Endonucleases: Quality Control
MSDS:Download MSDS as PDF
References:Dedkov, V.S., Belichenko, O.A., Gonchar, D.A., Akishev, A.G., Yamkovaya, T.V., Mezentseva, N.V., Tomilova, J.E., Abdurashitov, M.A., Degtyarev, S.K. Unpublished observations(2005)
Theoretical diagrams of DNA digestion by this enzyme for the most known DNA substrates:

To view the fragments length values please point mouse cursor over diagram
Fragment lengths
M12345M

M - ladder, 1 - Adeno-2 DNA, 2 - Lambda DNA, 3 - T7 DNA, 4 - pUC19, 5 - pBR322