|Source||An E. coli strain , that carries the cloned gene from Pseudomanas alcaligenes BS17|
|Assayed on||Lambda DNA|
|Unit definition||One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl.|
|Optimal SE-buffer||Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)|
|Enzyme activity (%)|
|25 - 50||10 - 25||0||0||100||40|
|Storage conditions||10 mM Tris-HCl (pH 7.5); 100 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C.|
|Ligation||After 5-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and 95% of these can be recut.|
|Non-specific hydrolisis||No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 10 u.a. of enzyme for 16 hours at 37°C.
|Reagents Supplied with Enzyme||
10 X SE-buffer Y|
|Methylation sensitivity||Blocked by CG methylation.|
|Inactivation 20 minutes under||65oC|
|Quality control||Restriction Endonucleases: Quality Control|
|MSDS:||Download MSDS as PDF|
|References:||Dedkov, V.S., Belichenko, O.A., Gonchar, D.A., Akishev, A.G., Yamkovaya, T.V., Mezentseva, N.V., Tomilova, J.E., Abdurashitov, M.A., Degtyarev, S.K.