Recognition site | ACTGGG(N)5↑ TGACCC(N)4↓ |
Source | Bacillus megaterium S87 |
Assayed on | Lambda DNA (HindIII-digest) |
Unit definition | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (HindIII-digest) in 1 hour at 37°C in a total reaction volume of 50 μl. |
Optimal SE-buffer | Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) |
Enzyme activity (%) | B | G | O | W | Y | Rose | 75 - 100 | 75 - 100 | 25 - 50 | 10 - 25 | 100 | 25 |
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Optimal temperature | 37oC |
Storage conditions | 10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol. Store at -20°C |
Ligation | After 2-fold overdigestion with enzyme about 75% of the DNA fragments can be ligated and 95% may be recut. |
Non-specific hydrolisis | Overnight digest with BmuI is not recommended. A 50 μl reaction containing 1 μg of Lambda DNA and 0.5 units of enzyme incubated for 4 hours resulted in the same pattern of DNA bands as a reaction incubated for 1 hour.
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Reagents Supplied with Enzyme |
10 X SE-buffer Y |
Methylation sensitivity | not tested |
Inactivation 20 minutes under | 65oC |
Notes | Enzyme is active in presence of EDTA. |
Quality control | Restriction Endonucleases: Quality Control |
MSDS: | Download MSDS as PDF |