Product info: Bmu I


Name
Bmu I
Cat. #E487E488
Package, u.a.50250
Concentration, u.a./ml500-1000500-1000

Recognition site
ACTGGG(N)5
TGACCC(N)4
SourceBacillus megaterium S87
Assayed onLambda DNA (HindIII-digest)
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA (HindIII-digest) in 1 hour at 37°C in a total reaction volume of 50 μl.
Optimal SE-bufferY (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)
Enzyme activity (%)
BGOWYRose
75 - 10075 - 10025 - 5010 - 2510025
Optimal temperature
37oC
Storage conditions10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol. Store at -20°C
LigationsAfter 2-fold overdigestion with enzyme about 75% of the DNA fragments can be ligated and 95% may be recut.
Non-specific hydrolisisOvernight digest with BmuI is not recommended. A 50 μl reaction containing 1 μg of Lambda DNA and 0.5 units of enzyme incubated for 4 hours resulted in the same pattern of DNA bands as a reaction incubated for 1 hour.
Reagents Supplied with Enzyme 10 X SE-buffer W, BSA
Methylation sensitivitynot tested
Inactivation 20 minutes under
65oC
NotesEnzyme is active in presence of EDTA.
Quality control
Restriction Endonucleases: Quality Control
MSDS:Download MSDS as PDF
Theoretical diagrams of DNA digestion by this enzyme for the most known DNA substrates:

To view the fragments length values please point mouse cursor over diagram
Fragment lengths
M12345M

M - ladder, 1 - Adeno-2 DNA, 2 - Lambda DNA, 3 - T7 DNA, 4 - pUC19, 5 - pBR322