Product info: Mal I


Name
Mal I
Cat. #E489E490
Package, u.a.50250
Concentration, u.a./ml500-1000500-1000

Recognition site
G(mA)TC
CT(mA)G
SourceMarinococus albus I
Substrate specificityThe enzyme cleaves only methylated DNA.
Assayed onpBR322 DNA (dam-methylated)
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of pBR322 DNA (dam-methylated) in 1 hour at 37°C in a total reaction volume of 50 μl.
Optimal SE-bufferSE-buffer MalI (20 mM Tris-HCl (pH 9.0 at 25°C); 10 mM MgCl2; 150 mM NaCl; 1 mM DTT.)
Enzyme activity (%)
BGOWYRose
25 - 5025 - 5050 - 7575 - 10050 - 7550
Reaction bufferSE-buffer MalI, (20 mM Tris-HCl (pH 9.0 at 25°C); 10 mM MgCl2; 150 mM NaCl; 1 mM DTT.)
Optimal temperature
37oC
Storage conditions10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA, 50% glycerol; Store at -20C.
LigationsAfter 2-fold overdigestion with enzyme more than 80% of the DNA fragments can be ligated and recut.
Non-specific hydrolisisNo nonspecific activity was detected after incubation of 1 μg of DNA with 2 u.a. of enzyme for 16 hours at 37C.
Reagents Supplied with Enzyme 10 X SE-buffer MalI
Methylation sensitivityThe enzyme cleaves only methylated DNA.
Inactivation 20 minutes under
65oC
MSDS:Download MSDS as PDF