|Assayed on||Adenovirus-2 DNA|
|Unit definition||One unit of the enzyme is the amount required to hydrolyze 1 μg of Adenovirus-2 DNA in 1 hour at 55°C in a total reaction volume of 50 μl.|
|Optimal SE-buffer||Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)|
|Enzyme activity (%)|
|75 - 100||50 - 75||10 - 25||25 - 50||100||100|
|Storage conditions||10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol. Store at -20°C.|
|Ligation||After 5-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.|
|Non-specific hydrolisis||No nonspecific activity was detected after incubation of 1 μg of Ad2 DNA with 5 u.a. of enzyme for 16 hours at 55°C.
|Reagents Supplied with Enzyme||
10 X SE-buffer Y|
|Methylation sensitivity||Not blocked by overlapping Dam-methylation (GmATC): GCGATCGC|
Blocked by CpG methylation.
|Inactivation 20 minutes under||80oC|
|Notes||High enzyme concentration may result in star activity.|
|Quality control||Restriction Endonucleases: Quality Control|
|MSDS:||Download MSDS as PDF|