Product info: Rga I Name Rga I    Cat. # E491 E492 Package, u.a. 200 1000 Concentration, u.a./ml 5000-10000 5000-10000 Recognition site GCGAT↑CGC CGC↓TAGCG Source Rhizoblum galegae Assayed on Adenovirus-2 DNA Unit definition One unit of the enzyme is the amount required to hydrolyze 1 μg of Adenovirus-2 DNA in 1 hour at 55°C in a total reaction volume of 50 μl. Optimal SE-buffer Y (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) Enzyme activity (%) B G O W Y Rose 75 - 100 50 - 75 10 - 25 25 - 50 100 100 Optimal temperature 55oC Storage conditions 10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol. Store at -20°C. Ligation After 5-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut. Non-specific hydrolisis No nonspecific activity was detected after incubation of 1 μg of Ad2 DNA with 5 u.a. of enzyme for 16 hours at 55°C. Reagents Supplied with Enzyme 10 X SE-buffer Y Methylation sensitivity Not blocked by overlapping Dam-methylation (GmATC): GCGATCGC Blocked by CpG methylation. Inactivation 20 minutes under 80oC Notes High enzyme concentration may result in star activity. Quality control Restriction Endonucleases: Quality Control MSDS: Download MSDS as PDF