Product info: Rga I


Name
Rga INot blocked by 
GCG(6mA)TCGC methylation  
Cat. #E491E492
Package, u.a.2001000
Concentration, u.a./ml5000-100005000-10000

Recognition site
GCGATCGC
CGCTAGCG
SourceRhizoblum galegae
Assayed onAdenovirus-2 DNA
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Adenovirus-2 DNA in 1 hour at 55°C in a total reaction volume of 50 μl.
Optimal SE-bufferY (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.)
Enzyme activity (%)
BGOWYRose
75 - 10050 - 7510 - 2525 - 50100100
Optimal temperature
55oC
Storage conditions10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 50% glycerol. Store at -20°C.
LigationsAfter 5-fold overdigestion with enzyme more than 90% of the DNA fragments can be ligated and recut.
Non-specific hydrolisisNo nonspecific activity was detected after incubation of 1 μg of Ad2 DNA with 5 u.a. of enzyme for 16 hours at 55°C.
Reagents Supplied with Enzyme 10 X SE-buffer Y
Methylation sensitivityNot blocked by overlapping Dam-methylation (GmATC): GCGATCGC
Blocked by CpG methylation.
Inactivation 20 minutes under
80oC
NotesHigh enzyme concentration may result in star activity.
Quality control
Restriction Endonucleases: Quality Control
MSDS:Download MSDS as PDF
Theoretical diagrams of DNA digestion by this enzyme for the most known DNA substrates:

To view the fragments length values please point mouse cursor over diagram
Fragment lengths
M12345M

M - ladder, 1 - Adeno-2 DNA, 2 - Lambda DNA, 3 - T7 DNA, 4 - pUC19, 5 - pBR322