Product info: M.Fsp4H I


Name
M.Fsp4H I
Cat. #M001
Package, u.a.100
Concentration, u.a./ml500-1000

Recognition site
G(m5C)NGC
SourceAn E.coli strain, that carries the cloned M.Fsp4HI gene from Flavobacterium species 4H.
DescriptionM.Fsp4HI Methyltransferase modifies the internal cytosine residue (C5) in the recognition sequence forming 5`-G(5mC)NGC-3`/3`-CGN(5mC)G-5`.
Unit definitionOne unit of the enzyme is the amount required to protect 1 μg of Lambda DNA in 1 hour at 30°C in a total reaction volume of 20 μl against cleavage by Fsp4HI restriction endonuclease.
Reaction bufferSE-buffer B + SAM, (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 1 mM DTT + 0,08 mM SAM.
(32 mM S-adenosylmethionine supplied))
Optimal temperature
30oC
Storage conditions10 mM Tis-HCl (pH 7.6); 100 mM NaCl; 0.1 mM EDTA; 10 mM 2-mercaptoethanol; 50% glycerol. Store at -20C.
NotesTo obtain 100% activity, SAM should be added to the 1x reaction mix to a final concentration of 0,08 mM.
MSDS:Download MSDS as PDF
References:Chmuzh E.V., Kashirina Yu.G., Tomilova Yu.E., Chernukhin V.A., Okhapkina S.S., Gonchar D. A., Dedkov V.S., Abdurashitov M. A., Degtyarev S. Kh. Gene cloning, comparative analysis of the protein structures from Fsp4HI restriction-modification system and biochemical characterization of the recombinant DNA methyltransferase M.Fsp4HI. // Molecular Biology, V.41, No 1, pp 43-50 (2007) (In Russian)