Product info: M.HspA I


Name
M.HspA I
Cat. #M003
Package, u.a.100
Concentration, u.a./ml1000-3000

Recognition site
G(5mC)GC
SourceAn E.coli strain, that carries the cloned M.HspAI gene from Haemophilus species AI.
DescriptionM.HspAI Methyltransferase modifies the internal cytosine residue (C5) in the recognition sequence forming 5`-G(5mC)GC-3`/3`-CG(5mC)G-5`.
Unit definitionOne unit of the enzyme is the amount required to protect 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 20μl against cleavage by HspAI restriction endonuclease.
Reaction bufferSE-buffer B + SAM, (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 1 mM DTT + 0,08 mM SAM.
(32 mM S-adenosylmethionine supplied))
Optimal temperature
37oC
Storage conditions10 mM Tis-HCl (pH 7.4); 0.1 mM EDTA; 10 mM 2-mercaptoethanol; 100 mM NaCl; 50% glycerol. Store at -20C.
NotesTo obtain 100% activity, SAM should be added to the 1x reaction mix to a final concentration of 0,08 mM.
MSDS:Download MSDS as PDF