Product info: Aco I


Name
Aco IBlocked by 
(5mc)YGGCCR methylation   
Cat. #E499E500
Package, u.a.100500
Concentration, u.a./ml500-2000500-2000

Recognition site
YGGCCR
RCCGGY
SourceAcinetobacter calcoaceticus
Assayed onLambda DNA(dam-, dcm-)
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA(dam-, dcm-) in 1 hour at 37°C in a total reaction volume of 50 μl.
Optimal SE-bufferG (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 50 mM NaCl; 1 mM DTT.)
Enzyme activity (%)
BGOWYRose
50 - 7510050 - 7525 - 5075 - 100100
Optimal temperature
37oC
Storage conditions10 mM Tris-HCl (pH 7.5); 200 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 μg/ml BSA; 50% glycerol; Store at -20°C.
LigationsAfter 3-fold overdigestion with AcoI more than 90% of Lambda DNA fragments can be ligated with T4 DNA Ligase at 16°C and recut.
Non-specific hydrolisisNo nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 2 u.a. of AcoI for 16 hours at 37°C.
Reagents Supplied with Enzyme 10 X SE-buffer G
Methylation sensitivityBlocked by overlapping dcm methylation (CmCWGG): CCTGGCCR.
Inactivation 20 minutes under
65oC
Quality control
Restriction Endonucleases: Quality Control
MSDS:Download MSDS as PDF
References:Chernukhin, V.A., Belichenko, O.A., Tomilova, J.E., Dedkov, V.S., Doroganov, A.O.. Unpublished observations. (2006)
Theoretical diagrams of DNA digestion by this enzyme for the most known DNA substrates:

To view the fragments length values please point mouse cursor over diagram
Fragment lengths
M12345M

M - ladder, 1 - Adeno-2 DNA, 2 - Lambda DNA, 3 - T7 DNA, 4 - pUC19, 5 - pBR322