Product info: FaeI


Name
FaeInew package  Blocked by C(6mA)TG methylation  
Cat. #E495E496
Package, u.a.50250
Concentration, u.a./ml500-2000500-2000

Recognition site
CATG
GTAC
SourceFlavobacterium aquatile N3
Assayed onpUC19 DNA
Unit definitionOne unit of the enzyme is the amount required to hydrolyze 1 μg of pUC19 DNA in 1 hour at 37°C in a total reaction volume of 50 μl.
Optimal SE-bufferSE-buffer FaeI (33 mM Tris-acetate (pH 8.3 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) + BSA
Enzyme activity (%)
BGOWYRose
25 - 5050 - 7510 - 2510 - 2575 - 100100
Optimal temperature
37oC
Storage conditions10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA; 50% glycerol; Store at -20°C.
LigationsAfter 2-fold overdigestion with enzyme more then 90% of the DNA fragments can be ligated with T4 DNA Ligase at 16°C and recut.
Non-specific hydrolisisNo nonspecific activity was detected after incubation of 1 μg of pUC19 DNA with 1 u.a. of enzyme for 16 hours at 37°C.
Reagents Supplied with Enzyme 10 X SE-buffer FaeI, BSA
Methylation sensitivityBlocked by CmATG methylation
Inactivation 20 minutes under
65oC
NotesTo obtain 100% activity, BSA should be added the 1x reaction mix to a final concentration of 100 μg/ml.
Do not use BSA for long incubation.
Quality control
Restriction Endonucleases: Quality Control
MSDS:Download MSDS as PDF
References:Chernukhin, V.A., Kileva, E.V., Tomilova, J.E., Dedkov, V.S.. Unpublished observations (2006).
Theoretical diagrams of DNA digestion by this enzyme for the most known DNA substrates:

To view the fragments length values please point mouse cursor over diagram
Fragment lengths
M12345M

M - ladder, 1 - Adeno-2 DNA, 2 - Lambda DNA, 3 - T7 DNA, 4 - pUC19, 5 - pBR322