| Recognition site | GCTCTTCN↑
CGAGAAG(N)4↓ |
| Source | Planococcus citreus S |
| Assayed on | Lambda DNA |
| Unit definition | One unit of the enzyme is the amount required to hydrolyze 1 μg of Lambda DNA in 1 hour at 37°C in a total reaction volume of 50 μl. |
| Optimal SE-buffer | B (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 1 mM DTT.) |
| Enzyme activity (%) | | B | G | O | W | Y | Rose | | 100 | 50 - 75 | 0 - 10 | 0 - 10 | 75 - 100 | 50 |
|
| Optimal temperature | 37oC |
| Storage conditions | 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0,1 mM EDTA; 7 mM 2-mercaptoethanol; 200 μg/ml BSA and 50% glycerol; Store at -20°C. |
| Ligations | After 3-fold overdigestion with enzyme 90% of the DNA fragments can be ligated with T4 DNA Ligase at 16°C and 95% of these can be recut. |
| Non-specific hydrolisis | No nonspecific activity was detected after incubation of 1 μg of Lambda DNA with 1 u.a. of enzyme for 16 hours at 37°C.
|
| Reagents Supplied with Enzyme |
10 X SE-buffer B |
| Methylation sensitivity | not tested |
| Inactivation 20 minutes under | 65oC |
| Quality control | Restriction Endonucleases: Quality Control |
| MSDS: | Download MSDS as PDF |
| References: | Chernukhin, V.A., Kileva, E.V., Tomilova, J.E., Dedkov, V.S. Unpublished observations (2006).
|
Theoretical diagrams of DNA digestion by this enzyme for the most known DNA substrates:
To view the fragments length values please point mouse cursor over diagram |
M - ladder, 1 - Adeno-2 DNA, 2 - Lambda DNA, 3 - T7 DNA, 4 - pUC19, 5 - pBR322
|