| Source | Isolated from E.coli strain that carries the recombinant plasmids |
| Description | TaqSE DNA polymerase is complex mix of thermostable DNA polymerase that possesses a 5` -> 3` polymerase
activity, 3` -> 5` exonuclease (proofreading) activity and a double strand specific 5` -> 3` exonuclease activity.
It may increase yield of reaction product compare to Taq DNA polymerase.
Application: long high fidelity primer extension reaction. |
| Unit definition | One unit is the amount of enzyme required to incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 72°C.
Unit Assay Conditions: 1 x Taq-DNA-polymerase buffer, 200 μM dNTPs including [3H]-dTTP and 250 μg/ml activated calf thymus DNA.
|
| Reaction buffer | SE-buffer DNA polymerase Taq, (60 mM Tris-HCl (pH 8.5 at 25°C); 1.5 mM MgCl2; 25 mM KCl; 10 mM 2-mercaptoethanol; 0.1% Triton X-100.) |
| Optimal temperature | 72oC |
| Storage conditions | 20 mM Tris-HCl (pH 7.6); 100 mM KCl; 0.1 mM EDTA; 1 mM DTT; 0.5% Triton X-100; 50% glycerol. Store at -20°C. |
| Quality control | The enzyme is purified free of contaminating endonucleases and
exonucleases.
|
| MSDS: | Download MSDS as PDF |