Product info: TaqSE DNA polymerase


Name
TaqSE DNA polymerase
Cat. #E313E314
Package, u.a.2001000
Concentration, u.a./ml50005000

SourceIsolated from E.coli strain that carries the recombinant plasmids
DescriptionTaqSE DNA polymerase is complex mix of thermostable DNA polymerase that possesses a 5` -> 3` polymerase activity, 3` -> 5` exonuclease (proofreading) activity and a double strand specific 5` -> 3` exonuclease activity.
It may increase yield of reaction product compare to Taq DNA polymerase.
Application: long high fidelity primer extension reaction.
Unit definitionOne unit is the amount of enzyme required to incorporate 10 nmol of dNTP into acid-insoluble material in 30 minutes at 72C. Unit Assay Conditions: 1 x Taq-DNA-polymerase buffer, 200 M dNTPs including [3H]-dTTP and 250 g/ml activated calf thymus DNA.
Reaction bufferSE-buffer DNA polymerase Taq, (60 mM Tris-HCl (pH 8.5 at 25°C); 1.5 mM MgCl2; 25 mM KCl; 10 mM 2-mercaptoethanol; 0.1% Triton X-100.)
Optimal temperature
72oC
Storage conditions20 mM Tris-HCl (pH 7.6); 100 mM KCl; 0.1 mM EDTA; 1 mM DTT; 0.5% Triton X-100; 50% glycerol. Store at -20C.
Quality controlThe enzyme is purified free of contaminating endonucleases and exonucleases.
MSDS:Download MSDS as PDF